Immunohistochemical study of expression of several proto-oncogenes in cancer tissues from patients undergoing radiation therapy

对40例头颈部鳞状细胞癌术前和放疗过程中活检标本冰冻切片进行P53单抗(P53(Ab3)识别突变型和P53(Ab5)野生型)免疫组化染色。P53(Ab3)在放疗前和照射剂量4Gy10Gy20Gy时的阳性表达率分别为25.0%、36.4%、25.7%和13.8%。突变型P53蛋白的表达频率在放射敏感性较高的肿瘤中呈上升趋势。

Immunostaining using p53 monoclonal antibodies (p53 (Ab-3) recognizes mutant type and p53 (Ab-5) the wild type of p53 protein) was performed on frozen sections of biopsy specimens obtained before and during preoperative radiotherapy from 40 patients with head and neck squamous cell carcinoma. The positive staining rates of p53 (Ab-3) before radiotherapy and at radiation doses of 4Gy, 10Gy and 20Gy were 25.0%, 36.4%, 25.7% and 13.8%, respectively. The frequency of the expression of mutant type p53 protein tended to increase in rather radiosensi-tive tumors.