Development and clinical application of a predictive assay for radiotherapy using an immunohistochemical method

为了开发可靠的放射治疗预测测定方法，在放射治疗期间使用癌症组织进行了免疫组织化学研究。对 21 名头颈鳞状细胞癌患者术前和术前放疗期间获得的冰冻切片进行 Ki-67 单克隆抗体免疫染色。在 20Gy 辐射剂量下 Ki-67 标记率迅速下降的肿瘤与不良的临床结果相关。此外，对 19 名头颈部癌症患者的癌组织中纤连蛋白的表达和分布进行了检查。结果与放射治疗后患者的预后相关。未发现纤连蛋白表达或分布发生显着变化。

For a development of a reliable predictive assay for radiation therapy, an immunohistochemical study using cancer tissues during radiotherapy was performed. Immunostaining with Ki-67 monoclonal antibody was performed on frozen sections obtained before and during preoperative radiation therapy from 21 patients with head and neck squamous cell carcinoma. Tumors with rapidly decreased Ki-67 labeling rates at radiation doses of 20Gy were related to poor clinical outcome. Moreover, fibronectin expression and distribution were examined in cancer tissue from 19 patients with cancer of the head and neck regions. The results were correlated with the patients' prognosis after radiation therapy. No remarkable changes in the fibronectin expression or distribution were found.