Reactive oxygen species producing site in radiation-induced apoptosis of human peripheral T cells: Involvement of lysosomal membrane destabilization
辐射诱导的人外周 T 细胞凋亡中活性氧的产生位点:溶酶体膜不稳定的参与
基本信息
- 批准号:15591281
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In our previous studies, we have partly elucidated the mechanism of radiation-induced apoptosis of human peripheral T cells. The exact site of the ROS (reactive oxygen species) formation induced by irradiation has been so far unknown. Therefore, in this study, we investigated the site of ROS formation by utilizing MitoCapture H2DCFDA (succinimidyl ester of dichlorodihydrofluorescein diacetate), DAPI, and Lysosensor. Our results showed that ROS formation apparently originated in the mitochondria and/or lysosomes instead of in the neclei of irradiated T cells. Moreover, lysosomal swelling and deformity, possibly revealing lysosomal membrane instability, were observed at 1 h after 5 Gy irradiation of T cells. At 4 h after irradiatronof 5 Gy, increase of fluorescence around the lysosomes, possibly revealing lysosomal ruputure, was seen. Based on the above results, we concluded the possible existence of a new apoptotic cascade involving early lysosomal membrane destabilization in radiation-induced apoptotsis of human peripheral T cells. Therefore, possible involvement of lysosomal protease leakage caused by hydroxyl radical formation in lysosomes (possibly resulting in mitochondrial membrane destabilization) is considered to play an important role in radiation induced T cell apoptosis.
在我们以前的研究中,我们已经部分阐明了辐射诱导人外周血T细胞凋亡的机制。到目前为止,辐射诱导的活性氧簇(ROS)形成的确切位点尚不清楚。因此,在本研究中,我们利用MitoCapture H2 DCFDA(二氯二氢荧光素二乙酸酯的琥珀酰亚胺酯)、DAPI和Lysosensor研究了ROS形成的位点。我们的结果表明,活性氧的形成显然起源于线粒体和/或溶酶体,而不是在核辐射的T细胞。此外,在5戈伊照射T细胞后1 h观察到溶酶体肿胀和变形,可能揭示了溶酶体膜的不稳定性。5戈伊照射后4 h,可见溶酶体周围荧光增强,可能为溶酶体损伤。基于上述结果,我们得出结论,可能存在一个新的凋亡级联反应,涉及早期溶酶体膜不稳定的辐射诱导的人外周血T细胞凋亡。因此,溶酶体中羟基自由基形成引起的溶酶体蛋白酶泄漏(可能导致线粒体膜不稳定)的可能参与被认为在辐射诱导的T细胞凋亡中起重要作用。
项目成果
期刊论文数量(41)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Prevention of hydrogen peroxide-induced apoptosis of human peripheral T cells by a lysosomotropic iron chelator, ammonium..
通过溶酶体铁螯合剂铵预防过氧化氢诱导的人外周 T 细胞凋亡。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:小川 恭弘;小川 恭弘;小川 恭弘
- 通讯作者:小川 恭弘
Reactive oxygen species-producing site in radiation-induced apoptosis of human peripheral T cells : Involvement of lysosomal.
辐射诱导的人外周 T 细胞凋亡中活性氧的产生位点:溶酶体的参与。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Ogawa Y.;et al.;小川 恭弘
- 通讯作者:小川 恭弘
小川恭弘: "Comparison of radiation-induced reactive oxygen species formation in adult articular chondrocytes and that in human peripheral T cells"Int.J.Mol.Med.. 11. 455-459 (2003)
Yasuhiro Okawa:“成人关节软骨细胞和人外周 T 细胞中辐射诱导的活性氧形成的比较”Int.J.Mol.Med.. 11. 455-459 (2003)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Immunocytochemical characteristics of human osteosarcoma cell line HS-Os-1 : Possible implication in apoptotic resistance...
人骨肉瘤细胞系 HS-Os-1 的免疫细胞化学特征:对细胞凋亡抵抗的可能影响...
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:小川 恭弘;小川 恭弘
- 通讯作者:小川 恭弘
Prevention of hydrogen peroxide-induced apoptosis of human peripheral T cells by a lysosomotropic iron chelator, ammonium.
通过溶酶体铁螯合剂铵预防过氧化氢诱导的人外周 T 细胞凋亡。
- DOI:
- 发表时间:2004
- 期刊:
- 影响因子:0
- 作者:Ogawa Y.;et al.;小川 恭弘;小川 恭弘;小川 恭弘
- 通讯作者:小川 恭弘
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OGAWA Yasuhiro其他文献
Transliteration from Uighur to Uzbek for Expansion of Japanese Translation Dictionary
从维吾尔语到乌兹别克语的音译,用于扩展日语翻译词典
- DOI:
- 发表时间:
2008 - 期刊:
- 影响因子:0
- 作者:
市橋歩実;松村真宏;田辺稔規;當麻俊介;松村 真宏;Toyoaki Nishida (Ed.);OGAWA Yasuhiro - 通讯作者:
OGAWA Yasuhiro
Design and Development of Japanese Translation Database System
日语翻译数据库系统的设计与开发
- DOI:
- 发表时间:
2011 - 期刊:
- 影响因子:0
- 作者:
TOYAMA Katsuhikoa;SAITO Daichi;SEKINE Yasuhiro;OGAWA Yasuhiro;KAKUTA Tokuyasu;KIMURA Tariho;MATSUURA Yoshiharu - 通讯作者:
MATSUURA Yoshiharu
OGAWA Yasuhiro的其他文献
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{{ truncateString('OGAWA Yasuhiro', 18)}}的其他基金
Automatic generation of the Outlines of Japanese Statutes
自动生成日本法规概要
- 批准号:
17K00460 - 财政年份:2017
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Relationship between spring water and slope failures occurred in a volcanic hillslope terrain under heavy rainfall
强降雨条件下火山山坡地形泉水与边坡破坏的关系
- 批准号:
23651175 - 财政年份:2011
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Statistical inflectional processing of agglutinative languages
粘着语言的统计屈折处理
- 批准号:
22700143 - 财政年份:2010
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Identification of the therapeutic effect of new enzyme-targeting radiosensitization treatment, KORTUC for tumor stem cells
新型酶靶向放射增敏治疗KORTUC对肿瘤干细胞的治疗效果鉴定
- 批准号:
21591610 - 财政年份:2009
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
ADAM22 localization mechanism and myelin formation at the Axon.
ADAM22 定位机制和轴突髓磷脂形成。
- 批准号:
21890269 - 财政年份:2009
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Research Activity Start-up
Research of music scale of Konkiok and its history in Taisau
康基奥音乐音阶及其在泰绍的历史研究
- 批准号:
20520344 - 财政年份:2008
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Development of Machine Translation system from Japanese into Uzbek
开发从日语到乌兹别克语的机器翻译系统
- 批准号:
18700141 - 财政年份:2006
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Identification of a new apoptotic pathway of radiation-induced apoptosis of human peripheral lymphocytes
辐射诱导人外周淋巴细胞凋亡新途径的鉴定
- 批准号:
18591382 - 财政年份:2006
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Robust Incremental Parsing based on Finite-State Approximation of Context Free Grammar
基于上下文无关文法有限状态逼近的鲁棒增量解析
- 批准号:
15300044 - 财政年份:2003
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Mechanism of radiation-induced apoptosis of human peripheral T cells
辐射诱导人外周T细胞凋亡的机制
- 批准号:
13670948 - 财政年份:2001
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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