Mechanism of radiation-induced apoptosis of human peripheral T cells

辐射诱导人外周T细胞凋亡的机制

• 批准号: 13670948
• 负责人: OGAWA Yasuhiro
• 金额: $ 2.3万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670948/
• 关键词: Lymphocyte; T cell; Apoptosis; Radiation; Mitochondria

The mechanism leading to the high level of radiosensitivity of T lymphocytes has not yet been fully described. In our previous study, we demonstrated that human peripheral T lymphocytes revealed early apoptotic changes (annexin-V-positive) and late apoptotic changes (propidium iodide-positive), at 13 and 24 h after irradiation of 5 Gy, respectively. Changes in mitochondrial membrane potential were observed at 10 h after irradiation of 5 Gy. Mitochondrial cytochrome c release was confirmed following the changes in mitochondrial membrane potential. In order to elucidate the mechanism which occurs prior to the mitochondrial membrane potential changes, we examined the radiation dose and the timing of oxidative DNA damage induced in human peripheral T lymphocytes following 10 MV X-ray irradiation. As a result, the production of 8-oxoguanine, i. e., the product of oxidative DNA damage, was clearly identified starting at 10, 6, and 3 h, after 2, 5, and 20 Gy of irradiation, respectively.

导致T淋巴细胞高水平放射敏感性的机制尚未完全描述。在我们以前的研究中，我们证明，人外周血T淋巴细胞显示早期凋亡的变化（膜联蛋白V阳性）和晚期凋亡的变化（碘化丙啶阳性），在13和24小时后，5戈伊照射，分别。5戈伊照射后10 h观察线粒体膜电位的变化。线粒体细胞色素c的释放被证实在线粒体膜电位的变化。为了阐明发生在线粒体膜电位变化之前的机制，我们研究了10 MV X射线照射后人外周血T淋巴细胞诱导的DNA氧化损伤的辐射剂量和时间。因此，生产8-氧代鸟嘌呤，即。例如，氧化性DNA损伤的产物，分别在2、5和20戈伊照射后10、6和3小时开始被清楚地识别。

项目成果

戸田巌雄: "The role of apoptotic resistance in irradiated adult articular chondrocytes"Int. J. Mol. Med.. 9. 349-352 (2002)

Iwao Toda：“凋亡抵抗在受辐射的成人关节软骨细胞中的作用”Int. Mol. Med.. 9. 349-352 (2002)

高橋敏明: "Role of apoptosis inhibition in various chondrocyte culture systems"Int. J. Mol. Med.. 11. 29-303 (2003)

Toshiaki Takahashi：“细胞凋亡抑制在各种软骨细胞培养系统中的作用”Int. J. Mol. 11. 29-303 (2003)

Ogawa Y, et al.: "Mitochondrial cytochrome c release in radiation-induced apoptosis of human peripheral T cells"Int J Mol Med.. 10. 263-268 (2002)

小川 Y 等：“辐射诱导的人外周 T 细胞凋亡中的线粒体细胞色素 c 释放”Int J Mol Med.. 10. 263-268 (2002)

Takahashi T, et al.: "Role of apoptosis inhibition in various chondrocyte culture systems"Int J Mol Med.. 11. 299-303 (2003)

Takahashi T 等人：“各种软骨细胞培养系统中细胞凋亡抑制的作用”Int J Mol Med.. 11. 299-303 (2003)

小林俊博: "スーパーオキサイドの細胞内検出法"細胞. 33. 272-275 (2001)

Toshihiro Kobayashi：“细胞内超氧化物的检测方法”Cell. 33. 272-275 (2001)。