Differential expression between proliferative and differentiative suclones from the acute myelomonoblastic leukemia cell line(ME-1)

急性粒细胞白血病细胞系(ME-1)增殖型和分化型suclon之间的差异表达

• 批准号: 06672310
• 负责人: SHIOSAKA Takahiko
• 金额: $ 1.34万
• 依托单位: Ehime College of Health Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672310/
• 关键词: ME-1; differential display; TPA; proliferation; differentiation; fyrosine kinase; tyrosine kinase; Leucine jipper; ATP; GTP結合モチーフ; tryosine kinase

Using the differentical display method, we examined differential geneexpression between two subclones, ME-F2 and ME-F3, which exhibit a proliferation and differentiation response to CSF and are derived from th acute myelomonoblastic leukemia cell line(ME-1).Three unknown genes (4-27 (81), 3-3-2, 4-18) were detected that are preferentially expressed in ME-F3 cells and upregulated in ME-F2 cells induced to differentiate by TPA.Two genes, stathmin and an unknown 3-7 gene, were detected that are preferentially expressed in ME-F2 cells and downreguleted in induced ME-F2 cells. The 3-7 gene encodes 807 amino acids (deduced from cDNA sequence) with a leucine zipper motif. Simultaneously, we examined protein tyrosinekinases expressed in this subclones. hyl, csk, tiel, tyk2 and c-fes were preferentially expressed in ME-F2 cells fibroblast growth factor was preferentially expressed in ME-F3 cells. Another unknown gene (1-6) preferentially expressed in ME-F2 cells showed similar expression to hyl, csk-between subclones and during induction to differentially in ME-F2 cells by TPA.These results demonstrate that differential gene expression in these subclones reflects proliferation and differentiation. Three unknown genes (4-27 (81), 3-3-2, 4-18) seem to correlate with differentiation and two unknowm genes (3-7, 1-6) seem to correlate with proliferation.

应用差异显示技术，我们检测了来自急性髓单核细胞白血病细胞系（ME-1）的两个亚克隆ME-F2和ME-F3的差异基因表达，这两个亚克隆对CSF具有增殖和分化反应，（4-27（81），3-3-2，4-18）的两个基因，stathmin和未知的3-7基因，在ME-F2细胞中优先表达，在诱导的ME-F2细胞中下调。3-7基因编码807个氨基酸（从cDNA序列推断），具有亮氨酸拉链基序。同时，我们检测了这些亚克隆中表达的蛋白酪氨酸激酶。ME-F2细胞中hyl、csk、蒂尔、tyk 2和c-fes优先表达; ME-F3细胞中成纤维细胞生长因子优先表达。另一个在ME-F2细胞中优先表达的未知基因（1-6）在亚克隆间和TPA诱导ME-F2细胞分化过程中的表达与hyl、csk-相似，表明这些亚克隆的差异表达反映了细胞的增殖和分化。三个未知基因（4-27（81）、3-3-2、4-18）似乎与分化相关，两个未知基因（3-7、1-6）似乎与增殖相关。

项目成果

Takahiko Shiosaka: "Abnormal responce of c-myc expression in bone marrow cells from myelodysplastic syndrone patients to plorhol ester" J. Exp. clinical cancer research. 13. 125-130 (1994)

Takahiko Shiosaka：“骨髓增生异常综合征患者骨髓细胞中 c-myc 表达对聚氯酚酯的异常反应”J. Exp。

高岡 洋子: "急性骨髄単球性白血病細胞株(ME-1)の増殖型および分化型サブクローン間における遺伝子発現の比較" 愛媛医学. (発売予定). (1996)

高冈洋子：“急性粒单核细胞白血病细胞系（ME-1）增殖和分化亚克隆之间的基因表达比较”爱媛医疗（1996年）。

塩坂孝彦: "白血病細胞における異常発現遺伝子の解析" 愛媛県立医療技術短期大学紀要. (発表予定). (1996)

Takahiko Shiosaka：“白血病细胞中异常表达基因的分析”爱媛县立医疗技术大学通报（待出版）（1996年）。

塩坂 孝彦: "白血病細胞における異常発言遺伝子の解析" 愛媛県立医学技術短期大学紀要. (発売予定). (1996)

Takahiko Shiozaka：“白血病细胞中异常表达基因的分析”爱媛医学技术大学通报（待发布）（1996年）。

T.Shiosaka, Y.Tanaka, S.Fujita: "Abnormal response of c-myc expression in bone marrow cells from Myelodysplastic syndrome Patients to Phorobol ester" J.Exp. clin. Cancer Res. 13. 125-129 (1994)

T.Shiosaka、Y.Tanaka、S.Fujita：“骨髓增生异常综合征患者的骨髓细胞中 c-myc 表达对 Phorobol 酯的异常反应”J.Exp。