Polymorphism of cytochrome P450IIE1 gene, alcohol elimination rate, and their application to forensic medicine

我们研究了CYP2E1基因多态性对酒精患者和健康受试者乙醇清除率的影响。采用聚合酶链反应-限制性片段长度多态性法对124名酗酒者和54名健康者的CYP2E1基因型进行了检测。酗酒者与健康对照者CYP2E1基因频率差异无统计学意义。入院时65名酗酒者的血乙醇浓度范围为0.32至4.22 mg/ml。cl/c2基因型患者的清除率与血乙醇浓度显著相关。在CYP2E1的三种基因型中，根据乙醇浓度将患者分为三组。血液中乙醇浓度大于2.5 mg/ml的c1/c2患者的平均乙醇消除率显著高于其他两组c1/c2患者。当血乙醇浓度大于2.5 mg/ml时，c1/c2患者的清除率明显高于c1/c1患者。无论CYP2E1基因型如何，当血液乙醇水平低于1.0 mg/ml时，酗酒者的清除率高于对照组。这些结果表明，在高乙醇水平下，CYP2E1的c2等位基因可能影响乙醇的消除速度。

We studied an influence of genetic polymorphisms in the CYP2E1 gene on ethanol elimination rate in alcoholic patients and healthy subjects. The CYP2E1 genotype was determined by polymerase chain reaction-restriction fragment length polymorphism method for 124 alcoholics and 54 healthy subjects. There was no significant difference in the gene frequency of CYP2E1 between the alcoholics and the healthy control subjects. Blood ethanol concentrations in the 65 alcoholics on admission ranged from 0.32 to 4.22 mg/ml. In the patients with thE cl/c2 genotype the elimination rate was significantly correlated with blood ethanol concentration. In each of the three genotypes of CYP2E1, the patients were divided into three groups based on ethanol concentrations. The average of ethanol elimination rate in the patients with c1/c2 having blood ethanol levels of more than 2.5 mg/ml was significantly higher than the rates in the two other groups of c1/c2. When blood ethanol levels were more than 2.5 mg/ml, the elimination rate in the patients with c1/c2 was significantly higher than that in those with c1/c1.Regardless of the CYP2E1 genotype, the elimination rate in the alcoholics was higher than that in the control subjects when blood ethanol levels were less than 1.0 mg/ml.These results suggest the possibility that the c2 allele of CYP2E1 influence the rate of ethanol elimination at high ethanol levels.