Polymorphism of cytochrome P450IIE1 gene, alcohol elimination rate, and their application to forensic medicine

细胞色素P450IIE1基因多态性、酒精消除率及其在法医学中的应用

• 批准号: 06807042
• 负责人: ADACHI Junko
• 金额: $ 1.28万
• 依托单位: kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06807042/
• 关键词: CYP2E1 genotype; alcoholics; ethanol elimination; ethanol intake; アルコール症患者; チトクロームP450IIE_1

We studied an influence of genetic polymorphisms in the CYP2E1 gene on ethanol elimination rate in alcoholic patients and healthy subjects. The CYP2E1 genotype was determined by polymerase chain reaction-restriction fragment length polymorphism method for 124 alcoholics and 54 healthy subjects. There was no significant difference in the gene frequency of CYP2E1 between the alcoholics and the healthy control subjects. Blood ethanol concentrations in the 65 alcoholics on admission ranged from 0.32 to 4.22 mg/ml. In the patients with thE cl/c2 genotype the elimination rate was significantly correlated with blood ethanol concentration. In each of the three genotypes of CYP2E1, the patients were divided into three groups based on ethanol concentrations. The average of ethanol elimination rate in the patients with c1/c2 having blood ethanol levels of more than 2.5 mg/ml was significantly higher than the rates in the two other groups of c1/c2. When blood ethanol levels were more than 2.5 mg/ml, the elimination rate in the patients with c1/c2 was significantly higher than that in those with c1/c1.Regardless of the CYP2E1 genotype, the elimination rate in the alcoholics was higher than that in the control subjects when blood ethanol levels were less than 1.0 mg/ml.These results suggest the possibility that the c2 allele of CYP2E1 influence the rate of ethanol elimination at high ethanol levels.

我们研究了 CYP2E1 基因的遗传多态性对酗酒患者和健康受试者的乙醇消除率的影响。采用聚合酶链反应-限制性片段长度多态性方法对124名酗酒者和54名健康受试者进行CYP2E1基因型测定。酗酒者和健康对照者之间的CYP2E1基因频率没有显着差异。 65 名酗酒者入院时的血液乙醇浓度范围为 0.32 至 4.22 mg/ml。在thE cl/c2基因型患者中，消除率与血液乙醇浓度显着相关。在 CYP2E1 的三种基因型中，根据乙醇浓度将患者分为三组。血液乙醇水平超过2.5 mg/ml的c1/c2组患者的平均乙醇消除率显着高于其他两组c1/c2组。当血液乙醇水平大于2.5 mg/ml时，c1/c2患者的消除率显着高于c1/c1患者。无论CYP2E1基因型如何，当血液乙醇水平低于1.0 mg/ml时，酗酒者的消除率均高于对照组。这些结果表明CYP2E1的c2等位基因可能影响CYP2E1的c2等位基因的消除率。 高乙醇浓度下的乙醇消除。

项目成果

上野易弘: "CYP2E1遺伝子がアルコール代謝速度に及ぼす影響" アルコールと医学生物学. 15. 37-42 (1995)

Yoshihiro Ueno：“CYP2E1 基因对酒精代谢率的影响”《酒精与医学生物学》15. 37-42 (1995)。

上野 易弘: "CYP2E1遺伝子がアルコール代謝速度に及ぼす影響" アルコールと医学生物学. 15. 37-42 (1995)

Yoshihiro Ueno：“CYP2E1 基因对酒精代谢率的影响”《酒精与医学生物学》15. 37-42 (1995)。

Y.Ueno: "Effect of cytochrome P450IIE1 genotype on ethanol elimination rate in alcoholics and control subjects" Alcoholism Clin.Exp.Res.20. (1996)

Y.Ueno：“细胞色素 P450IIE1 基因型对酗酒者和对照受试者乙醇消除率的影响”Alcoholism Clin.Exp.Res.20。