Involvement of beta-carbolines in the symptoms of alcohol withdrawal

β-咔啉与酒精戒断症状的关系

• 批准号: 02807066
• 负责人: ADACHI Junko
• 金额: $ 1.02万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02807066/
• 关键词: beta-carboline; alcoholism; alcoholic beverage; alcohol drinking; alcohol; acetaldehyde; βーカルボリン; アルコ-ル摂取; アルコ-ル; トリプトファン; アルコ-ル症; アルコ-ル飲料

Tetrahydro-beta-carboline-3-carboxylic acid, a condensation reaction product of acetaldehyde, a metabolite of alcohol, with tryptophan can be considered a probable precursor of the benzodiazepine receptor antagonist, beta-carboline-3-carboxylate. beta-Carboline (norharman) and 1-methyl-beta-carboline (harman) have been prepared by the reaction of trytophan with some aldehydes under an oxidative condition. It is well known that these beta-carbolines inhabit monoamine oxidase and benzodiazepine receptor binding.In the present study, a purification procedure, involving a chemically-bonded material followed by HPLC combined with fluorometric detection, was used for the quantitative determination of tetraphydro-beta-carbolines. An HPLC and mass spectrometry method was also developed for their identification. Norharman and harman in various alcoholic beverages could be detected, although the contents were very small. Moderate amounts of tetrahydro-beta-carboline-3-carboxylic acid were found in brewed alcoholic bevarage, however, distillled spilits contained low concentration.Next, Urinary excretions of tetrahydro-beta-carbolines were measured in man after administration of ethanol (0.4g/kg). Blood ethanol and acetaldehyde levels were determined by gas chromatography. Ethanol intake caused increased excretion of tetrahydro-beta-carboline-3-carboxylic acid, though there was no significant correlation between blood acetaldehyde and urinary excretion of tetrahydro-beta-carbolines.

四氢-β-咔啉-3-羧酸是乙醇代谢物乙醛与色氨酸的缩合反应产物，可被认为是苯二氮卓类受体拮抗剂β-咔啉-3-羧酸的可能前体。色氨酸与醛在氧化条件下反应，合成了β-咔啉（norharman）和1-甲基-β-咔啉（harman）。众所周知，这些β-咔啉抑制单胺氧化酶和benzodiazepine受体binding.In本研究中，纯化程序，涉及一个化学键合的材料，然后通过HPLC结合荧光检测，用于定量测定四氢-β-咔啉。HPLC和质谱法也被开发用于鉴定它们。诺哈曼和哈曼在各种酒精饮料中都能检测到，虽然含量很小。四氢-β-咔啉-3-羧酸在酿造酒饮料中含量中等，而在蒸馏酒饮料中含量较低。血液中乙醇和乙醛的水平通过气相色谱法测定。乙醇的摄入导致四氢-β-咔啉-3-羧酸的排泄增加，尽管血液乙醛和四氢-β-咔啉的尿排泄之间没有显著的相关性。

项目成果

Junko ADACHI et al: "Endogenous formation of tetrahydro-beta-carboline and effect of ethanol consumption on the urinary excretion" Alcohol Metabolism and the Liver. 11. 123-127 (1992)

Junko ADACHI 等人：“四氢-β-咔啉的内源性形成以及乙醇消耗对尿液排泄的影响”酒精代谢和肝脏。

足立順子 他: "アルコール飲料及び食品中のβ-カルボリン(変異原物質)" アルコール代謝と肝. 10. 129-133 (1991)

Junko Adachi 等人：“酒精饮料和食品中的 β-咔啉（诱变剂）”《酒精代谢和肝脏》10. 129-133 (1991)。