Elucidation of molecular structure of lipid peroxides related to biomembrane injury and application of those to legal medicine

生物膜损伤相关脂质过氧化物分子结构的阐明及其在法医学中的应用

• 批准号: 14370155
• 负责人: ADACHI Junko
• 金额: $ 8.13万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370155/
• 关键词: HPLC-CL; liver-disorder; PCOOH; lipid peroxidation; alcohol; oxysterol; paraquat; alcoholic patients; フリーラジカル; リン脂質過酸化物; LC-MS; アルコール依存症患者; 肝機能検査値; コレステロール過酸化物; パラコート中毒; アルコール; オキシステロール; ラット

1.Molecular structure of membrane lipid peroxidesUsing LC/APCI-MS, 7-ketocholesterol and 7-hydroxycholesterol were identified in skeletal muscle and kidney of rats. Phosphatidyocholine (PC) monohydroperoxide (PC 16:0/18:2-OOH and PC 18:0/18:2-OOH) were identified in rat heart and human plasma by LC/ESI-MS.2.Quantitative analyses of oxysterol by HPLC-UV and PCOOH by HPLC-CL were developed.3.Accumulation of oxysterols in lung of victims with paraquat poisoning was associated with pulmonary fibrosis. Consecutive administration of paraquat to rats induced enhanced cholesterol peroxidation and lung injury.4.Chronic ethanol feeding induced accumulation of oxysterols in skeletal muscle and liver. Plasma PCOOH is elevated in alcoholic patients with liver disease suggest the involvement of lipid peroxidation in alcohol-induced liver damage.Thus, we have shown not only oxysterol but PCOOH are prominent markers of oxidative stress.

1.用LC/APCI-MS技术，在大鼠骨骼肌和肾脏中鉴定出7-酮胆固醇和7-羟基胆固醇。采用LC/ESI-MS法测定了大鼠心脏和人血浆中的磷脂酰胆碱（PC）单氢过氧化物（PC 16：0/18：2-OOH和PC 18：0/18：2-OOH）。百草枯连续染毒可引起大鼠胆固醇过氧化反应增强和肺损伤。4.慢性乙醇喂养可引起大鼠骨骼肌和肝脏中氧化固醇的积累。酒精性肝病患者血浆PCOH升高提示脂质过氧化参与了酒精性肝损伤，因此，我们发现不仅氧固醇而且PCOH都是氧化应激的重要标志物。

项目成果

Chronic ethanol feeding increases 7-hydroperoxycholesterol and oxysterols in rat skeletal muscle.

长期喂食乙醇会增加大鼠骨骼肌中的 7-氢过氧胆固醇和氧化甾醇。

• 发表时间: 2002
• 期刊: Metabolism 51巻・6号
• 作者: Tetsuo Fujita

Comprehensive handbook of alcohol-related pathology Vol.3 Chapter 95

酒精相关病理学综合手册第3卷第95章

• 发表时间: 2004
• 作者: Junko Adach

Early differential gene expression of rat lung after exposure to paraquat

百草枯暴露后大鼠肺早期差异基因表达

• 发表时间: 2004
• 期刊: Free Radical Res 38巻・8号
• 作者: 廣田良夫;Masafumi Tomita
• 通讯作者: Masafumi Tomita

H Ariyoshi: "Effect of chronic ethanol feeding on oxysterols in rat liver"Free Radical Research. 36巻6号. 661-666 (2002)

H Ariyoshi：“慢性乙醇喂养对大鼠肝脏中氧甾醇的影响”自由基研究，第 36 卷，第 661-666 期（2002 年）。

J Adachi: "Fatty acid profile in skeletal muscle of the rat in response to acute (2.5 hours) and prolonged(6 weeks) ethanol-dosage"Addiction Biology. 第8巻. 181-189 (2003)

J Adachi：“大鼠骨骼肌中的脂肪酸对急性（2.5 小时）和长期（6 周）乙醇剂量的反应”《成瘾生物学》第 8 卷，181-189 (2003)。