Elucidation of molecular structure of lipid peroxides related to biomembrane injury and application of those to legal medicine

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1.Molecular structure of membrane lipid peroxidesUsing LC/APCI-MS, 7-ketocholesterol and 7-hydroxycholesterol were identified in skeletal muscle and kidney of rats. Phosphatidyocholine (PC) monohydroperoxide (PC 16:0/18:2-OOH and PC 18:0/18:2-OOH) were identified in rat heart and human plasma by LC/ESI-MS.2.Quantitative analyses of oxysterol by HPLC-UV and PCOOH by HPLC-CL were developed.3.Accumulation of oxysterols in lung of victims with paraquat poisoning was associated with pulmonary fibrosis. Consecutive administration of paraquat to rats induced enhanced cholesterol peroxidation and lung injury.4.Chronic ethanol feeding induced accumulation of oxysterols in skeletal muscle and liver. Plasma PCOOH is elevated in alcoholic patients with liver disease suggest the involvement of lipid peroxidation in alcohol-induced liver damage.Thus, we have shown not only oxysterol but PCOOH are prominent markers of oxidative stress.