Melecular mechanism of cdholesterol hydroperoxides as indices membrane disorder and its application to forensic medicine

1. 通过分析胆固醇源性过氧化氢（7α -00H和7β -00H）作为脂质过氧化的指标，研究了百草枯诱导的体内氧化应激。大鼠ig百草枯10mg/kg。在肾脏和肝脏中，在2h发现脂质过氧化增强。肾脏持续升高5天。这似乎反映了急性低剂量百草枯处理大鼠肾脏病理中更大的氧化应激。酒精性肌病我们测试了在酒精暴露的初始阶段可以识别肌肉的乙醇敏感性的假设。为了验证这一点，我们测量了比目鱼和跖肌中的胆固醇氢过氧化物。2.5 h后，乙醇单独增加了植物肌肉的7α 00H和7β -00H。24小时后，两种氢过氧化物在每块肌肉中均升高。这些结果表明II型肌肉比I型肌肉更敏感。CO中毒我们测量了大鼠大脑中的胆固醇氢过氧化物，这些大鼠分别接受假手术（对照组）、缺氧或吸入CO (1005ppm) 90分钟，然后恢复48小时。缺氧组7α-00H和7β-00H较低，而一氧化碳组与对照组比较无明显变化。这表明一氧化碳对大脑的损伤是通过脂质过氧化作用而独立于缺氧。

1. Paraquat poisoningThe in vivo paraquat-induced oxidative stress was studied by analyzing cholesterol-derived hydroperoxide (7α -00H and 7β -00H) as an index of lipid peroxidation. Paraquat (10mg/kg) was administered i.p. to rats. In kidney and liver, augumented lipid peroxidation was found at 2h. The increase remained in kidney 5 days. It seems to reflect greater oxidative stress in the pathology of kidney of rats treated with acute paraquat at low dose.2. Alcoholic myopathyWe tested the hypotheses that ethanol sensitivities of muscle can be discerned in the initial periods of ethanol exposure. To test this we measured cholesterol hydroperoxides in soleus and plantaris muscle. After 2.5 h, ethanol increased 7α 00H and 7β -00H in plantaris muscle alone. After 24 h both hydroperoxides wefe elevated in each muscle. These results suggest that Type II muscle is sensitive than Type I muscle.3. CO poisoningWe measured cholesterol hydroperoxides in the brains of rats that underwent either a sham operation (control), hypoxia, or CO inhalation (1005 ppm) for 90 min followed by 48 h of recovery. 7α-00H and 7β-00H were low in the hypoxia group, while these were unaltered in the CO group compared with the controls. It suggests that CO injures to the brain through lipid peroxidation independently of hypoxia.