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Melecular mechanism of cdholesterol hydroperoxides as indices membrane disorder and its application to forensic medicine

胆甾醇氢过氧化物作为膜紊乱指标的分子机制及其在法医学中的应用

基本信息

批准号：
12670394
负责人：
ADACHI Junko
金额：
$ 2.24万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670394/
关键词：
cholesterol hydroperoxide free radical lipid peroxidation paraquat alcohol CO ラット一酸化炭素

项目摘要

1. Paraquat poisoningThe in vivo paraquat-induced oxidative stress was studied by analyzing cholesterol-derived hydroperoxide (7α -00H and 7β -00H) as an index of lipid peroxidation. Paraquat (10mg/kg) was administered i.p. to rats. In kidney and liver, augumented lipid peroxidation was found at 2h. The increase remained in kidney 5 days. It seems to reflect greater oxidative stress in the pathology of kidney of rats treated with acute paraquat at low dose.2. Alcoholic myopathyWe tested the hypotheses that ethanol sensitivities of muscle can be discerned in the initial periods of ethanol exposure. To test this we measured cholesterol hydroperoxides in soleus and plantaris muscle. After 2.5 h, ethanol increased 7α 00H and 7β -00H in plantaris muscle alone. After 24 h both hydroperoxides wefe elevated in each muscle. These results suggest that Type II muscle is sensitive than Type I muscle.3. CO poisoningWe measured cholesterol hydroperoxides in the brains of rats that underwent either a sham operation (control), hypoxia, or CO inhalation (1005 ppm) for 90 min followed by 48 h of recovery. 7α-00H and 7β-00H were low in the hypoxia group, while these were unaltered in the CO group compared with the controls. It suggests that CO injures to the brain through lipid peroxidation independently of hypoxia.

1.百草枯中毒通过分析胆固醇衍生的氢过氧化物（7α -00H和7β -00H）作为脂质过氧化的指标，研究了百草枯诱导的体内氧化应激。对大鼠腹腔注射百草枯（10 mg/kg）。在肾脏和肝脏中，在2 h发现脂质过氧化作用增强。肾脏中的增加持续5天。这可能反映了低剂量急性百草枯处理大鼠肾脏病理中氧化应激作用的增强.酒精性肌病我们检验了肌肉对酒精的敏感性在酒精暴露的最初阶段就可以辨别的假设。为了验证这一点，我们测量了比目鱼肌和跖肌中的胆固醇氢过氧化物。2.5h后，乙醇单独增加跖肌中的7α 00 H和7β -00H。24小时后，两种过氧化氢均在每块肌肉中升高。这些结果表明II型肌比I型肌敏感. CO中毒我们测量了接受假手术（对照）、缺氧或CO吸入（1005 ppm）90分钟后恢复48小时的大鼠脑中胆固醇氢过氧化物。与对照组相比，低氧组7α-00 H和7β-00 H降低，而CO组7α-00 H和7β-00 H无变化。提示一氧化碳通过脂质过氧化损伤脑组织，与缺氧无关。