Molecular mechanisms of the membrane disorder in the senile dementia due to free radical

脑血管型老年性痴呆是由自由基相关的脂质过氧化作用引起的脑缺血性损伤。首先，我们研究了胆固醇氢过氧化物的鉴定和定量方法。接下来，我们在尸检时收集了痴呆症患者和健康受试者的脑组织。然后我们将其分为六个区域，分别是额叶皮层和白色物质、顶颞叶皮层和白色物质、枕叶皮层和白色物质。将每个组织均质化并提取脂质。采用高效液相色谱-质谱联用技术对7-羟基胆固醇、7-酮基胆固醇和5，6-环氧胆固醇进行了定性分析。此外，我们定量7 α和7 β氢过氧胆固醇的高效液相色谱荧光检测。我们发现7-氢过氧胆固醇在老年痴呆症的大脑中积累。在脑区的顶颞部白色物质中蓄积明显。

Senile dementia of cerebrovascular type is caused by ischemic injury of brain due to free radical-related lipid peroxide. First of all we investigated the methods for identification and quantification of cholesterol hydroperoxide. Next, we collected brain tissue of dementia and healthy subjects at autopsy. Then we divided into six regions, frontal cortex and white matter, parietotemporal cortex and white matter, and occipital cortex and white matter. Each tissue was homogenized and lipid was extracted. We identified 7-hydroperoxycholestero l 7-hydroxycholesterol, 7-ketocholesterol, and 5,6-epoxycholesterol by high performance liquid chromatography-mass spectrometory. In addtion we quantified 7 alpha -and 7- beta hydroperoxycholesterol by high performance liquid chromatography with chemiluminescent detection. We found an accumulation of 7-hydroperoxycholesterol in the brain of senile dementia. The accumulation was evident in parietotemporal white matter among the brain regions.