Synthesis, transport and fate of G-protein coupled 7-transmembrane receptors

G蛋白偶联7次跨膜受体的合成、转运和命运

• 批准号: 06808079
• 负责人: YAMADA Hisao
• 金额: $ 1.15万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06808079/
• 关键词: G protein coupled; 7 transmembrane; receptor; intracellular transport; cell membrane; GnRH; endothelin; neuron; Gタンパク; ET‐A受容体; Gプロテイン共役型; 膜7回貫通型; 膜組み込み経路; GnRH; エンドセリン; カテコールアミン

The synthesis, transport and fate of G-protein coupled 7-transmembrane receptors can be analyzed using neuron model. Because a neuron has perikaryon where receptor protein is synthesized, and long processes where receptors are functioning.The hypothalamus and mesencephalon of adult male Wistar rats were immunostained with the antibodies to GnRH receptor and endothelin type A receptor, and observed under light-, electron- and conforcal laser scan microscopes. The consumption or internalization of these receptors was analyzed by ligand administration. In these experiments, the Golgi-budding and axonal transport were inhibited by cerebroventricular injection of brefeldin A and colchicine, respectively. Moreover, we observed time-course change of immunostaining pattern of ET-A receptor in comparison with catecholamine synthesizing enzyme, TH and DBH,in the 60HDA administered rat.We conclude that this type receptor is synthesized in the rER-Golgi system, incorporated into the membrane, and then transported with vesicular membrane from Golgi-budding to fusion with the cell membrane.

G 蛋白偶联 7 次跨膜受体的合成、转运和命运可以使用神经元模型进行分析。因为神经元有合成受体蛋白的核周，以及受体发挥功能的长突。用 GnRH 受体和内皮素 A 型受体的抗体对成年雄性 Wistar 大鼠的下丘脑和中脑进行免疫染色，并在光、电子和共聚焦激光扫描显微镜下观察。通过配体施用来分析这些受体的消耗或内化。在这些实验中，脑室注射布雷菲德菌素 A 和秋水仙碱分别抑制高尔基体出芽和轴突运输。此外，我们观察了给予60HDA的大鼠中ET-A受体与儿茶酚胺合成酶、TH和DBH的免疫染色模式的时程变化。我们得出结论，这种类型的受体在rER-高尔基体系统中合成，掺入膜中，然后通过囊泡膜从高尔基体出芽运输到与细胞膜融合。

项目成果

H. Yamada,J Ochi: "Ditribution of GnRH receptor in mouse brain" Neuroscience Research. 19. S60 (1994)

H. Yamada、J Ochi：“小鼠大脑中 GnRH 受体的分布”神经科学研究。

H.Yamada, J.Ochi: "Histocytochemical and functional aspects on the "Brain-Vascular Peptides"" Acta Anatomica Nipponica. 70 (5). 422-435 (1995)

H.Yamada、J.Ochi：““脑血管肽”的组织细胞化学和功能方面”《日本解剖学报》。

T.Nishimura,H.Yamada,M.Kinoshita,J.Ochi: "Endothelin expression during rat heart development:An immunohistochemical and in situ hybridization study" Biomedical Research. 15(5). 291-298 (1994)

T.Nishimura、H.Yamada、M.Kinoshita、J.Ochi：“大鼠心脏发育过程中内皮素的表达：免疫组织化学和原位杂交研究”生物医学研究。

T. Nishimura,H. Yamada,M. Kinoshita,J. Ochi: "Endothelin expression during rat heart development : An immunohistochemical and in situ hybridization study" Biomedical Research. 15(5). 291-298 (1994)

T.西村，H.

T.Nishimura, H.Yamada, M.Kinoshita, J.Ochi: "Endothelin expression during rat heart development : An imunohistochemical and in situ hybridaization study" Biomedical Research. 15 (5). 291-298 (1994)

T.Nishimura、H.Yamada、M.Kinoshita、J.Ochi：“大鼠心脏发育过程中的内皮素表达：免疫组织化学和原位杂交研究”生物医学研究。