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Histocytochemical Analysis for New Vasoactive Substances in CNS

中枢神经系统中新血管活性物质的组织细胞化学分析

基本信息

批准号：
01570030
负责人：
YAMADA Hisao
金额：
$ 1.34万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

Recently, some humoral agents which play a role in water-balance, electrolyte metabolism and blood pressure control, i. e. ANP/BNP/CNP family (ANPs), endothelin (ET) and endogenous digitalis-like substance (EDLS) were identified and their chemical stucture determined. In this research, we analyzed these vasoactive substances with a histocytochemical technique using the central nervous system of rat, pig, dog and macaque.The nerve terminals of ANP- and BNP-neurons were observed in the posterior horn of the spinal cords of rats and pigs. The BNP-neurons were seen in the pig hypothalamus, but were not found in the rat brain except the spinal cord. These results were confirmed by biochemical and physiological reports, which proposed that BNP existed mainly in the mammalian heart but not in the brain ; and CNP, a new peptide of ANPs is found in mammalian brain.ET-immunoreactivities were not recognized in the mammalian central nervous system. The central nervous tissues have 10^2pg/g of ET, … More which level of bioactive substance cannot be detected with immunohistochemistry, though there were some immunohistochemical reports using the mammalian brain. In those reports, the researcher used the antibody, the epitope which had high homology in nelation to other bioactive substances. We made a ontogenetical study on this neuropeptide, i. e. ET in the rat with immunohistochemical and molecular biological technique. ET was first detected on day 19 of gestation in a salivary gland. Because ET is also considered to be a cell-proliferation factor, ET is possible to be the secondary discovered NGF (nerve growth factor).EDLS were histochemically studied using anti-digoxin and anti-ouabain antibodies which have highーaffinity to EDLS. EDLS-neurons were distributed in the magnocellular nuclei of mammalian hypothalamus. Recent biochemical study shows EDLS resembles to ouabain, cis-trans-cis type steroid of plant origin. It is thought that cis-trans-cis type steroid exists in the mammalian brain as a neurohormone. Less

近年来，一些在水平衡、电解质代谢和血压控制中起作用的体液因子，如：e.对ANP/BNP/CNP家族（ANP）、内皮素（ET）和内源性洋地黄样物质（EDLS）进行鉴定和化学结构测定。本研究利用大鼠、猪、狗和猕猴的中枢神经系统，采用组织细胞化学技术对这些血管活性物质进行了分析，观察了大鼠和猪脊髓后角ANP和BNP神经元的神经末梢。在猪的下丘脑中可见到BNP神经元，而在大鼠的脑中除脊髓外均未见到。这一结果得到了生化和生理学研究的证实，即BNP主要存在于哺乳动物的心脏而不存在于脑中; CNP是一种新的ANP肽，存在于哺乳动物的脑中; ET-免疫反应在哺乳动物的中枢神经系统中不被识别。中枢神经组织ET含量为10^2pg/g， ...更多信息尽管有一些使用哺乳动物脑的免疫组织化学报告，但用免疫组织化学不能检测生物活性物质的水平。在这些报告中，研究人员使用了抗体，即与其他生物活性物质具有高度同源性的表位。我们对这种神经肽进行了个体发生学研究。e.应用免疫组织化学和分子生物学技术检测大鼠血浆ET水平。在妊娠第19天首次在唾液腺中检测到ET。由于ET也被认为是一种细胞增殖因子，因此ET可能是第二个被发现的神经生长因子（NGF）。EDLS神经元分布于哺乳动物下丘脑的大细胞核。最近的生化研究表明，EDLS类似于哇巴因，顺-反-顺型植物源类固醇。据认为，顺-反-顺型类固醇作为神经激素存在于哺乳动物大脑中。少