Excessive-ramification and sprouting of intra-pancreatic sensory nerve-fibers in the chronic pancreatitis model.

慢性胰腺炎模型中胰腺内感觉神经纤维的过度分支和出芽。

基本信息

批准号：
16590157
负责人：
YAMADA Hisao
金额：
$ 1.98万
依托单位：
Kansai Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590157/
关键词：
axonal branching dorsal root ganglia neuronal tracing pain chronic pancreatitis WBN Kob rat immunohistochemistry behavioral study

项目摘要

Increase in number of intrapancreatic nerve bundles has been implicated in the generation of persistent pain in chronic pancreatitis. To examine the origin of these nerve fibers and the mechanisms linking neural morphological change to pain generation, we used neuronal tracing techniques in combination with immunohistochemistry in spontaneous chronic pancreatitis in the WBN/Kob rats.For retrograde tracing, horseradish peroxidase (HRP) was injected into the pancreas, and labeled neurons in the sensory ganglia were counted. For anterograde tracing, biotinylated dextran amine (BDA) was injected into the dorsal root ganglia (DRGs), and labeled intrapancreatic sensory fibers were histochemically assessed. For assessment of pain generation, we evaluated c-Fos-positive neurons in the spinal dorsal horn and behavioral changes of the animals.In WBN/Kob rats, numbers of HRP-labeled neurons were decreased in the DRGs, and numbers of BDA-labeled intrapancreatic nerve fibers and terminals were increased. BDA-labeled nerve fibers contained growth-associated protein-43 (GAP-43). The number of c-Fos-positive neurons in the dorsal horn was also increased and was correlated with intrapancreatic GAP-43-immunoreactivity. Grooming behavior was reduced in WBN/Kob rats and this reduction was facilitated by exocrine stimulation. Axonal branching in DRG neurons innervating the pancreas increases in WBN/Kob rats ; and these morphological changes are likely involved in pain generation in chronic pancreatitis.

癌间神经束的数量增加与慢性胰腺炎的持续疼痛产生有关。为了检查这些神经纤维的起源以及将神经形态变化与产生疼痛联系起来的机制，我们在WBN/KOB大鼠中使用神经元追踪技术与自发性慢性胰腺炎中的免疫组织化学结合使用。神经节被计数。为了进行顺行追踪，将生物素化的右旋胺（BDA）注入了背根神经节（DRG），并通过组织化学评估了标记的癌间内感觉纤维。为了评估疼痛产生的评估，我们评估了脊柱背角和动物的行为变化的C-FOS阳性神经元。在WBN/KOB大鼠中，DRG中的HRP标记神经元数量减少，BDA标记的内脑内神经纤维和末端的数量增加了。 BDA标记的神经纤维包含与生长相关的蛋白质-43（GAP-43）。背角中的C-FOS阳性神经元的数量也增加了，并且与综合性GAP-43-免疫反应性相关。 WBN/KOB大鼠的修饰行为降低了，通过外分泌刺激促进了这种降低。 DRG神经元中的轴突分支支配胰腺增加了WBN/KOB大鼠的增加；这些形态学的变化可能与慢性胰腺炎的疼痛产生有关。