Studies on structure and function of organelles dividing machines based on genome information

基于基因组信息的细胞器分裂机结构与功能研究

• 批准号: 14204078
• 负责人: KUROIWA Tsuneyoshi
• 金额: $ 36.52万
• 依托单位: Rikkyo University (2003-2005)The University of Tokyo (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14204078/
• 关键词: Cyanidioschyzon merolae; PD ring; plastid division; MD ring; mitochondrial division; FtsZ ring; organelle division; Cyanidioschyzon merolae(シゾ; 色素体分裂装置; ミトコンドリア分裂装置; ゲノム情報; TOF-MS; シゾン; ゲノム解析; 細胞核ゲノム; オルガネラ分裂装置; ミトコンドリア分裂・増殖; 葉緑体分裂・増殖; C

To elucidate the mechanism of mitochondria and plastid division under the control of the cell nucleus, we provided the primitive red alga Cyanidioschyzon merolae as a material. C.merolae cells have very advantageous for organelle divisions because the cell contains minimum number of organelles of which divisions were synchronized by light and dark cycles. The obtained data are compared with those of higher organisms. We first examined plastid dividing (PD) ring apparatus as the circumference of PD ring was 2.5 times longer than that of mitochondrial dividing (MD) ring apparatus. To obtain pure proteins from intact PD ring apparatus, we improved several points such as efficiency of synchronization of chloroplast division, isolation of pure dividing chloroplasts, isolation of intact PD ring apparatus by various detergent treatments and complete extraction of proteins from isolated intact PD ring apparatus. We then obtained SDS gel spots by using two dimensional electrophoresis and analyzed the spots by MALDI-TOFMS on basis of C.merolae genome information. We identified about 30 genes related to PD ring fractions and made their antibodies. We found at least novel 4 genes which are contained in PD ring apparatus and continue to find more genes related intimately to organelle divisions according to the established methods.

为了阐明线粒体和质体分裂在细胞核的控制下的机理，我们提供了原始的红色藻类Cyanidioschyzon Merolae作为一种材料。 C.Merolae细胞对细胞器分裂具有非常有利的优势，因为该细胞包含最小数量的细胞器，其中裂片通过光和深色循环同步。将获得的数据与较高生物体的数据进行比较。我们首先检查了质体分裂（PD）环形设备，因为PD环的周长比线粒体分裂（MD）环形设备长2.5倍。为了从完整的PD环形设备中获得纯蛋白质，我们提高了几个点，例如叶绿体分裂同步的效率，纯化的分裂叶绿体的分离，通过各种洗涤剂处理完整的PD环形设备，并完全从孤立的完整PD环形设备中萃取蛋白质。然后，我们通过使用二维电泳获得了SDS凝胶斑点，并根据C.Merolae基因组信息分析了Maldi-Tofms的斑点。我们确定了大约30个与PD环馏分相关的基因，并产生了抗体。我们发现至少有4种新的4个基因，这些基因包含在PD环形设备中，并继续根据已建立的方法与细胞器分裂密切相关的更多基因。

项目成果

Nishimura, Y., Misumi, S., et al.: "mt^+ gamate specific nuclease that targets mit-chloroplasts during the sexual reroduction in Chlamydomonas reinhardtii"Genes Dev.. 16. 1116-1128 (2002)

Nishimura，Y.，Misumi，S.等人：“在莱茵衣藻有性繁殖过程中靶向线粒体叶绿体的配子特异性核酸酶”Genes Dev.. 16. 1116-1128 (2002)

Improvement of cultures conditions and evidence for nuclear tranformation by homologous recombination in a red alga, Cyanidioschyzon merolae

通过红藻 Cyanidioschyzon merolae 中的同源重组改善培养条件和核转化证据

• 发表时间: 2004
• 期刊: Plant Cell Physiol. 45
• 作者: Nomaki;H.;Heinz;P.;Hemleben;C.;Kitazato;H.;Minoda et al.
• 通讯作者: Minoda et al.

Nishida, K., Takahara, M., et al.: "Dynamic recruitment of daynamin for final mitochondrial severance in a red alga"Proc.Natl.Acad.Sci.USA. (in press).

Nishida, K.、Takahara, M. 等人：“动态招募 Daynamin 以实现红藻中最终线粒体的切断”Proc.Natl.Acad.Sci.USA。

Inada, N., Sakai, A., et al.: "Three-dimensional progression of programmed death in the rice coleoptile"Int.Rev.Cytol.. 218. 221-253 (2002)

Inada, N., Sakai, A., et al.：“水稻胚芽鞘中程序性死亡的三维进程”Int.Rev.Cytol.. 218. 221-253 (2002)

The division of pleomorphic plastids with multiple FtsZ rings in tobacco BY-2 cells.

烟草 BY-2 细胞中具有多个 FtsZ 环的多形质体的分裂。

• 发表时间: 2003
• 期刊: Europ. J. Cell Biol. 82
• 作者: Momoyama;Y.
• 通讯作者: Y.