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CELL PHISIOLOGICAL AND MOLECULAR BIOLOGICA STUDIES ON THE DIVIDING APPARATUS OF PLASTIDS AND MITOCHONDRIA

质体和线粒体分裂装置的细胞生理学和分子生物学研究

基本信息

批准号：
10440236
负责人：
KUROIWA Tsuneyoshi
金额：
$ 10.37万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Plastidokinesis makes use of organelle dividing apparatuses such as plastid-dividing ring (PD ring) and mitochondrion-dividing ring (MD ring). The plastid-dividing apparatus (PD apparatus) is composed of three electron-dense rings (the outer, middle and inner), while the mitochondrion-dividing apparatus (MD apparatus) is triple electron-dense rings in cytoplasm, inter-space between inner and outer envelopes and inner ring in the mitochondrial matrix.The behaviors of both the PD and MD apparatuses throughout organelle division in Cyanidioschyzon merolae has been revealed in detail by electron microscopy. When cells enter mitosis, the inner PD ring forms first, followed by the outer and middle rings and finally the MD rings. The PD rings begin to contract before the MD rings. However, the MD rings start to contract at about 4 times the speed of the PD rings and catch up to the PD rings. The cross-sectional areas of both the outer PD and MD rings increase as contraction in the plane of division progress. This suggests that the outer rings of organelle dividing apparatuses (OD apparatus) provide the motive forse for contractian. Recently the dividing chloroplasts with a PD ring have been isolated from the cells and it is essential for isolation of the intact PD ring and identification of proteins in the PD ring. The FtsZ genes of both mitochondrial and plastid types were isolated from the cell and their antibodies were obtained from mouse. Immuno-electron microscopy using their antibodies showed that the FtsZ proteins were not located on the PD ring and the MD ring, respectively.

质体运动利用细胞器分隔装置，例如质体分隔环（PD 环）和线粒体分隔环（MD 环）。质体分裂装置（PD装置）由三个电子致密环（外环、中环和内环）组成，而线粒体分裂装置（MD装置）由细胞质中的三重电子致密环、内外包膜之间的空隙以及线粒体基质中的内环组成。 PD和MD装置在整个细胞器中的行为电子显微镜详细揭示了 Cyanidioschyzon merolae 的分裂。当细胞进入有丝分裂时，首先形成内PD环，然后是外环和中环，最后是MD环。 PD 环先于 MD 环开始收缩。然而，MD 环开始以 PD 环大约 4 倍的速度收缩，并赶上 PD 环。外 PD 环和 MD 环的横截面积随着分割平面收缩的进行而增加。这表明细胞器分裂装置（OD装置）的外环为收缩提供了动力。最近，已从细胞中分离出带有PD环的分裂叶绿体，这对于分离完整的PD环和鉴定PD环中的蛋白质至关重要。从细胞中分离出线粒体和质体类型的 FtsZ 基因，并从小鼠中获得其抗体。使用他们的抗体进行的免疫电子显微镜显示，FtsZ 蛋白分别不位于 PD 环和 MD 环上。