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CELL PHISIOLOGICAL AND MOLECULAR BIOLOGICA STUDIES ON THE DIVIDING APPARATUS OF PLASTIDS AND MITOCHONDRIA

质体和线粒体分裂装置的细胞生理学和分子生物学研究

基本信息

批准号：
10440236
负责人：
KUROIWA Tsuneyoshi
金额：
$ 10.37万
依托单位：
THE UNIVERSITY OF TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

Plastidokinesis makes use of organelle dividing apparatuses such as plastid-dividing ring (PD ring) and mitochondrion-dividing ring (MD ring). The plastid-dividing apparatus (PD apparatus) is composed of three electron-dense rings (the outer, middle and inner), while the mitochondrion-dividing apparatus (MD apparatus) is triple electron-dense rings in cytoplasm, inter-space between inner and outer envelopes and inner ring in the mitochondrial matrix.The behaviors of both the PD and MD apparatuses throughout organelle division in Cyanidioschyzon merolae has been revealed in detail by electron microscopy. When cells enter mitosis, the inner PD ring forms first, followed by the outer and middle rings and finally the MD rings. The PD rings begin to contract before the MD rings. However, the MD rings start to contract at about 4 times the speed of the PD rings and catch up to the PD rings. The cross-sectional areas of both the outer PD and MD rings increase as contraction in the plane of division progress. This suggests that the outer rings of organelle dividing apparatuses (OD apparatus) provide the motive forse for contractian. Recently the dividing chloroplasts with a PD ring have been isolated from the cells and it is essential for isolation of the intact PD ring and identification of proteins in the PD ring. The FtsZ genes of both mitochondrial and plastid types were isolated from the cell and their antibodies were obtained from mouse. Immuno-electron microscopy using their antibodies showed that the FtsZ proteins were not located on the PD ring and the MD ring, respectively.

质体分裂利用细胞器分裂装置，如质体分裂环（PD环）和质体分裂环（MD环）。本发明公开了一种分质体装置，（PD装置）由三个电子致密环组成（外，中，内），而分瓣器（MD装置）是细胞质中的三重电子致密环，表示“间”线粒体基质中内外被膜之间的空间和内环。通过对紫蓝雪鱼细胞器分裂过程中PD和MD装置的行为的研究，电镜当细胞进入有丝分裂时，内部PD环首先形成，然后是外部和中间环，最后是MD环。PD环在MD环之前开始收缩。然而，MD环开始以PD环的大约4倍的速度收缩并赶上PD环。外PD和MD环的横截面积随着分裂面的收缩而增加。这表明细胞器的外环（OD器）为收缩提供了动力。近年来，人们从细胞中分离出了具有PD环的分裂叶绿体，这对于分离完整的PD环和鉴定PD环中的蛋白质是必不可少的。从细胞中分离线粒体型和质体型的FtsZ基因，并从小鼠中获得它们的抗体。使用其抗体的免疫电子显微镜显示FtsZ蛋白分别不位于PD环和MD环上。