权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

A study of identification of the proteins and their genes which compose the dividing apparatus of mitochondria and plastids

构成线粒体和质体分裂装置的蛋白质及其基因的鉴定研究

基本信息

批准号：
12440222
负责人：
KUROIWA Tsuneyoshi
金额：
$ 10.82万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

The plastid-dividing ring can be detected by transmission electron microscopy as two (or three) electron-dense rings: and outer ring on the cytosolic face of the outer envelope, occasionally a middle ring in the intermembrane space, and an inner ring on the stromal face of the inner envelope. The FtsZ ring, which plays a central role in bacterial division, also is involved in plastid division and is believed to have descended to plastids from cyanobacterial endosymbiosis. The relationship between the two structures is not known, although there is discussion regarding whether they are identical, biochemical and immunocytochemical investigations, using synchronized chloroplasts of the red alga Cyanidoschyzon merolae, showed that the plastid FtsZ ring is distinct and separable from the plastid-dividing ring. The FtsZ ring localizes in stroma and faces the inner plastid-dividing ring at the far side from the inner envelope. The FtsZ ring and the inner and outer plastid-dividing ring form in that order before plastid division. The FtsZ ring disappears at the late stage of constriction before dissociation of the plastid-dividing ring, when the constriction is still in progress. Our results suggest that the FtsZ ring-based system, which originated from a plastid ancestor, cyanobacteria, and the plastid-dividing ring-based system, which probably originated from host eukaryotic cells, form a complex and are involved in plastid division by distinct modes.

质体分隔环可以通过透射电子显微镜检测为两个（或三个）电子致密环：外层环位于外膜的胞质面上，偶尔有一个中环位于膜间空间，内环位于内膜的基质面上。 FtsZ环在细菌分裂中起核心作用，也参与质体分裂，并且被认为是从蓝藻内共生下降到质体的。这两种结构之间的关系尚不清楚，尽管有关于它们是否相同的讨论，但使用红藻 Cyanidoschyzon merolae 的同步叶绿体进行的生化和免疫细胞化学研究表明，质体 FtsZ 环与质体分隔环不同且可分离。 FtsZ 环位于基质中，面向内包膜远端的内质体分隔环。 FtsZ 环以及内质体分裂环和外质体分裂环在质体分裂之前按该顺序形成。当缢缩仍在进行时，FtsZ 环在质体分隔环解离之前的缢缩后期消失。我们的结果表明，源自质体祖先蓝细菌的基于 FtsZ 环的系统和可能源自宿主真核细胞的基于质体分裂环的系统形成一个复合体，并通过不同的模式参与质体分裂。