Studies on dynamic changes of molecular distribution in plant cells by immuno-electron microscopy

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We have developed an immuno-light-electron microscopy and examined the dynamic changes of DNA and proteins in plant cells. As a result, we have obtained new data about these problems. Especially, Osafune and Ueda improved the immuno-gold-electron microscopy (IGEM) to obtain a high resolution. The newly developed techniques are applied to various fields of these investigators including the group. Kuroiwa developed a technique for the simultaneous observations of light microscopic field and electron microscopic field and applied the technique to analyzing the multiplication of cells in root meristems of Pelargonium zonale, Nicotiana tabacum and Arabidopsis thaliana. The results showed that the mtDNA content per mt-nucleus in the cells just above quiescent center, where the synthesis of mtDNA is active, corresponds to approximately 3000 kilobase pairs but, in the meristematic cells just below the elongation zone of the root it falls less than 170 kbp. Murakami showed using IGEM that the photosystem I is located in thylakoid membrane systems, while the photosystem II in stroma thylakoids. Osafune found by a high resolution IGEM that LHCPII apoprotein transferred to plastid through the Golgi apparatus. Ueda showed that the actin is located on the surface of lysosomes in Trebouxia. Tanaka, Yamaguchi and Miyakawa revealed the behavior of proteinase, the formation of virus-like particles and the existence of mitchondorial nuclear proteins in yeast cells, respectively.