Studies on total regulation system of plastid and mitochondrial division machineries

申请人已经发现了原始红藻和黏菌中线粒体和质体增殖所必需的细胞器分裂环（称为PD环和MD环）。之后，申请人开发了原始红球藻Cyanidioschyzon merolae的细胞器分裂的同步化、基因组核苷酸的100%测序、细胞器分裂机构的分离以及基因破坏和液相色谱法的方法。本工作的目的是利用这些技术来了解细胞器分裂机制的全貌。因此，我们发现了一个控制包括线粒体和质体在内的所有细胞器分裂的基因TOP，为阐明整个细胞的分裂过程提供了有益的线索。

The applicants discovered already organelle-dividing rings (named as PD ring and MD ring) essential for the proliferation of mitochondria and plastids in the primitive red algae and slime mold. After that, the applicant developed the synchronization of organelle divisions of the primitive red alga, Cyanidioschyzon merolae, the 100% sequencing of genome nucleotides, the isolation of the organelle-dividing machinery, and the methods of gene disruption and of the liquid chromatography. The aim of this work was to understandthe whole picture of the mechanism of organelle division by using these technologies. As a result, We have found a gene, TOP, that controls the division of all organelles including mitochondria and plastids and got the beneficial leads to elucidate the division of the whole cell.