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Development of Biological Sample Separation/Extraction and Preparation Microsystems for High Performance Biosensing

开发用于高性能生物传感的生物样品分离/提取和制备微系统

基本信息

批准号：
16201031
负责人：
SHOJI Shuichi
金额：
$ 32.2万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16201031/
关键词：
Micro Fluidic Systems Micro Total Analysis Systems MEMS Micro Fludic Devices Biomolecule Sorter Sample Injector Glass Microstructures Sheath Flow ガラス微細構造

项目摘要

In recent one of the most active area of Micro Total Analysis Systems (μTAS) or Lab-on-a-Chip (LOC), DNA, Protein analysis methods and devices have been improved. However, traditional sample preparations before analysis are required in many cases. In order to realize high speed and high throughput analysis in μTAS, sample separations/extractions are requested to be obtained in microfluidic devices/systems. We applied MEMS technologies and nano technologies and fabricated micro fluidic devices and systems of nl or pl in volume.Biomolecules sorting systems using so-gel transformation of the thermo-sensitive hydrogel as the carrier fluid were developed. High speed one inlet and two outlets sorter is realized by optimizing the channel structures and the flow behaviors. Very fast sorting within 5 msec was also realized. One inlet and 9 outlets sorting system is realized in a matrix microchannel using IR light illumination projected by the PC controlled DMD. A micro chamber integrated with an extremely low dead volume reagent injector for real-time monitoring of living cell was developed. In order to realize specific introduction of the reagent, we also developed the 3D sheath flow scanner. For high throughput analysis, a prototype of 8 diagnostic micro chambers having passive distributing facilitates simultaneous cell injection. On the other hand, microfluidic systems using sheath flow behavior in the microchannel for biological cell sorting were also developed. The flow system can sort two different cells to 16 outlet microchambers. To realize high efficient reaction of DNA and protein, two phase micro droplets of organic/water and three phase micro droplets of organic/water/air genelation fluidic devices were proposed.

近年来最活跃的领域之一是微量全分析系统（μTAS）或芯片实验室（LOC），DNA、蛋白质分析方法和设备得到了改进。然而，在许多情况下，分析前需要进行传统的样品制备。为了实现μTAS的高速、高通量分析，需要在微流控装置/系统中实现样品分离/提取。我们应用MEMS技术和纳米技术，制备了nl或pl体积的微流体器件和系统。开发了以热敏水凝胶的so-gel转化为载液的生物分子分选系统。通过优化通道结构和流动行为，实现高速一进二出分选机。还实现了 5 毫秒内的快速排序。使用 PC 控制的 DMD 投射的红外光照明在矩阵微通道中实现 1 个入口和 9 个出口的分选系统。开发了一种集成极低死体积试剂注射器的微室，用于实时监测活细胞。为了实现试剂的特异性引入，我们还开发了3D鞘流扫描仪。对于高通量分析，具有被动分布的 8 个诊断微室原型有助于同时进行细胞注射。另一方面，还开发了利用微通道中鞘流行为进行生物细胞分选的微流体系统。该流动系统可以将两种不同的细胞分选至 16 个出口微室。为了实现DNA与蛋白质的高效反应，提出了有机/水两相微滴和有机/水/空气三相微滴生成流体装置。