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Studies on sorting and fixing of. Bioiooiecules for high performance micro chemical/biochemical analysis systems

排序与固定的研究.

基本信息

批准号：
12450167
负责人：
SHOJI Shuichi
金额：
$ 8.38万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

Biomolecles handling microsystemsMicro biomolecules handling flow cell using 3-D sheath flow was fabricated. It uses the simple micro structure designed by MEMS CAD system. On the other hand, cross-linked methyl cellulose (MC) changes its state from liquid to gel by elevating the temperature up to 55 ℃. In order to realize micro flow control, we applied this phenomenon to realize micro flow switch for cell sorting. To achieve high speed sorting, we fabricated a PC controlled flow system consists of an automatic fluorescence sample detector, focused IR laser sorce, and two galvanometer mirrors for switching and scanning of the laser. The detection and switching time of 120 msec is achieved.Micro flow cell for multi-analyse bioaffinity assayA micro flow cell for the multi-analyse bioaffinity assay system was designed. It has two Teflon micro check values. Some different antibodies for clinical diagnostic were immobilized on center surface of the channel as bioaffinity probes, which realizes sequential multi-analyse assay. An efficient reaction with small sample volume is expected.Micro mixer/reactor using multilayer laminar flowAn efficient micromixer using nonhomogeneous multilaer in laminar flow regime was fabricated. To increase the mixing efficiency, the CFD simulation was used to optimize the width of the multilayers. It is estimated that the reagents mixing is proceeded much faster compared to previous multiplayer micro mixers.Metal electrodes implanted PDMS micro cellMetal electrodes or heaters implanted PDMS structures were proposed and fabricated. Prototype micro flow cell having two pairs of the parallel electrodes in order to control the electric field or the temperature is fabricated and evaluated.

生物分子处理微系统利用三维鞘流技术制备了微生物分子处理流动细胞。它采用MEMS CAD系统设计的简单微结构。另一方面，交联甲基纤维素（MC）通过将温度升高到55℃，从液体状态转变为凝胶状态。为了实现微流控制，我们利用这一现象实现微流开关进行细胞分选。为了实现高速分选，我们制作了一个PC控制的流量系统，该系统由一个自动荧光样品检测器、聚焦红外激光源和两个用于切换和扫描激光的振镜组成。实现了120毫秒的检测和切换时间。设计了一种多分析生物亲和性检测系统微流细胞。它有两个特氟龙微止回阀值。将临床诊断用的不同抗体作为生物亲和探针固定在通道中心表面，实现了序列多分析检测。期望在样品体积小的情况下进行有效的反应。采用多层层流技术制备了一种高效的多层非均匀层流微混合器。为了提高混合效率，采用CFD模拟对多层膜的宽度进行了优化。据估计，与以前的多人微型混合器相比，试剂混合速度要快得多。提出并制作了金属电极植入PDMS微电池。制作并评价了具有两对平行电极控制电场或温度的微流电池原型。