Studies on sorting and fixing of. Bioiooiecules for high performance micro chemical/biochemical analysis systems

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Biomolecles handling microsystemsMicro biomolecules handling flow cell using 3-D sheath flow was fabricated. It uses the simple micro structure designed by MEMS CAD system. On the other hand, cross-linked methyl cellulose (MC) changes its state from liquid to gel by elevating the temperature up to 55 ℃. In order to realize micro flow control, we applied this phenomenon to realize micro flow switch for cell sorting. To achieve high speed sorting, we fabricated a PC controlled flow system consists of an automatic fluorescence sample detector, focused IR laser sorce, and two galvanometer mirrors for switching and scanning of the laser. The detection and switching time of 120 msec is achieved.Micro flow cell for multi-analyse bioaffinity assayA micro flow cell for the multi-analyse bioaffinity assay system was designed. It has two Teflon micro check values. Some different antibodies for clinical diagnostic were immobilized on center surface of the channel as bioaffinity probes, which realizes sequential multi-analyse assay. An efficient reaction with small sample volume is expected.Micro mixer/reactor using multilayer laminar flowAn efficient micromixer using nonhomogeneous multilaer in laminar flow regime was fabricated. To increase the mixing efficiency, the CFD simulation was used to optimize the width of the multilayers. It is estimated that the reagents mixing is proceeded much faster compared to previous multiplayer micro mixers.Metal electrodes implanted PDMS micro cellMetal electrodes or heaters implanted PDMS structures were proposed and fabricated. Prototype micro flow cell having two pairs of the parallel electrodes in order to control the electric field or the temperature is fabricated and evaluated.