Spatio-temporal analysis of small GTPases involved in vesicular trafficking by fluorescence imaging

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Vesicular traffic regulates not only the process essential for cell survival but also intercellular and intracellular signaling. To elucidate the regulatory mechanism of vesicular traffic, we developed biosensors based on the principle of fluorescent resonance energy transfer, and succeeded for the first time to visualize the activity change of low-molecular weight GTPases on the vesicles. Furthermore, we identified molecules that regulate the activity of such low-molecular weight GTPases.