Analysis of DNA damage response using gene-disrupted DT40 clones.

同源重组（Homologous recombination， HR）在修复由喜树碱和顺铂等化疗药物引起的DNA损伤中起着重要作用。HR是通过50多种不同蛋白质的协调作用来完成的。我们通过破坏鸡DT40细胞中编码HR蛋白的基因，分析了单个HR蛋白的作用。我们已经揭示了以下HR蛋白的功能，包括CtIP、DNA聚合酶θ和ν、Fan1、Palb-2、Sfr1、Slx4和Sws1。

Homologous recombination (HR) plays a major role in repairing DNA damage caused by chemotherapeutic agents, such as camptothecin and cisplatin. HR is carried out by coordinate actions of more than 50 different proteins. We have analyzed the role of the individual HR proteins by disrupting genes encoding the HR proteins in the chicken DT40 cells. We have revealed the function of the following HR proteins, including CtIP, DNA polymerases θ and ν, Fan1, Palb-2, Sfr1, Slx4, and Sws1.