Identification of the essential factor(s) to establish the genome imprinting in mammals.

鉴定在哺乳动物中建立基因组印记的基本因素。

• 批准号: 21810010
• 负责人: MIYANO Masaru
• 金额: $ 1.76万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21810010/
• 关键词: 遺伝子; ゲノム刷り込み; 発現制御; クロマチン構造

In order to understand the mechanism of the genome imprinting, we tried to identify the factor(s), epigenetic modifications of chromatin, and higher order chromatin structures requiring for the establishment of the imprinting. We demonstrated that higher order chromatin loop structures at human H19 gene locus in the avian cell hybrids are different from those in mammalian cells, although DNA methylation status and histone modifications at the locus in the hybrids are maintained to those in the mammalian cells. This result suggested a possibility that it may be important to being formed the proper higher order chromatin structures mediated by an unknown factor that is not in the avian cells, but is in the mammalian cells.

为了了解基因组印记的机制，我们试图确定建立印记所需的因素、染色质的表观遗传修饰以及高阶染色质结构。我们证明，禽类细胞杂交体中人类 H19 基因位点的高阶染色质环结构与哺乳动物细胞中的不同，尽管杂交体中该位点的 DNA 甲基化状态和组蛋白修饰与哺乳动物细胞中的保持一致。这一结果表明，对于由不在鸟类细胞中但在哺乳动物细胞中的未知因子介导的正确的高级染色质结构的形成可能很重要。

项目成果

What is the essential component in the evolution of genomic imprinting in mammals.

哺乳动物基因组印记进化的重要组成部分是什么？

• 发表时间: 2009
• 作者: 宮野勝;堀家牧子;押村光雄;堀家慎一
• 通讯作者: 堀家慎一