Research on mechanism of a novel signal polypeptide-induced innate immune activation.

本研究的目的是阐明一种新的信号肽（N '-CARD-PTD）诱导天然免疫激活的机制和N'-CARD-PTD的佐剂作用。前期研究发现组蛋白H2 B参与dsDNA介导的先天免疫激活。最近我们合成了一种新的融合蛋白N '-CARD（nuclear localization signal of H2 B fused CARD of IPS-1），它能激活核DNA解旋酶II（NDH）介导的天然免疫应答。在本研究中，我们阐明了融合蛋白转导结构域的N '-CARD（N'-CARD-PTD）激活的树突状细胞依赖于I型IFN，而不是TLR。作为疫苗佐剂，N '-CARD-PTD强烈诱导针对各种抗原的抗原特异性抗体应答。

The aim of this research is to clarify mechanism of a novel signal polypeptide (N'-CARD-PTD)-induced innate immune activation and adjuvanticity of N'-CARD-PTD. Previous research we identified histone H2B is involved in dsDNA-mediated innate immune activation. Recently we synthesized the newly fusion protein, N'-CARD (nuclear localization signal of H2B fused CARD of IPS-1), activated nuclear DNA helicase II (NDH)-mediated innate immune responses. In this research, we clarify that protein transduction domain fused N'-CARD (N'-CARD-PTD) activated dendritic cells depend on type I IFN, but not TLRs. As a vaccine adjuvant, N'-CARD-PTD strongly induced antigen specific antibody response against various antigen.