Analysis on the higher-order chromatin structure in the interphase nucleus using super-resolution fluorescence microscopy

我开发了一种利用超分辨率显微镜3DSIM进行活体观察的方法，并应用于活体裂变酵母。活分裂酵母的间期染色质形成直径为100 ~ 150nm的纤维结构。用修饰组蛋白抗体对固定裂变酵母细胞核的染色质进行染色，用3DSIM观察，定量分析与DNA质量的重叠程度。结果表明，与正在进行的转录相关的区域被脱浓缩并从纤维结构中延伸。

I developed a method for live observation using super-resolution microscopy, 3DSIM, and applied on live fission yeast. The interphase chromatin in live fission yeast formed fibrous structures with a diameter of 100-150 nm. Chromatin in fixed fission yeast nuclei was stained with antibodies against modified histones and observed with 3DSIM, then the level of overlap with the DNA mass was quantitatively analyzed. The results showed that regions associated with ongoing transcription were de-condensed and extended from fibrous structures.