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Analysis on the higher-order chromatin structure in the interphase nucleus using super-resolution fluorescence microscopy

Analysis on the higher-order chromatin structure in the interphase nucleus using super-resolution fluorescence microscopy
使用超分辨率荧光显微镜分析间期核中的高阶染色质结构
批准号:
22870042
负责人:
MATSUDA Atsushi
金额:
$2.01万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Research Activity Start-up
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2011

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中文摘要
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英文摘要
I developed a method for live observation using super-resolution microscopy, 3DSIM, and applied on live fission yeast. The interphase chromatin in live fission yeast formed fibrous structures with a diameter of 100-150 nm. Chromatin in fixed fission yeast nuclei was stained with antibodies against modified histones and observed with 3DSIM, then the level of overlap with the DNA mass was quantitatively analyzed. The results showed that regions associated with ongoing transcription were de-condensed and extended from fibrous structures.
期刊论文(26)
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会议论文
DOI: 10.1371/journal.pone.0012768
发表时间: 2010-09-15
期刊: PloS one
影响因子: 3.7
作者: [Matsuda A, Shao L, Boulanger J, Kervrann C, Carlton PM, Kner P, Agard D, Sedat JW]
通讯作者: Sedat JW
超分解能顕微鏡で見る細胞核構造
用超分辨率显微镜观察细胞核结构
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [Satomi Kanno, Masato Yamawaki, AtsushiHirose, Hiroki Ishibashi, Natsuko Kbayashi, Keitaro Tanoi, Laurent Nussaume, Tomoko M. Nakanishi, 松田厚志]
通讯作者: 松田厚志
超分解能蛍光顕微鏡3DSIMにより見る間期核クロマチン
使用超分辨率荧光显微镜 3DSIM 观察间期核染色质
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [Hitomi Sawai, Hiroshi Sugimoto, Haruto Ishikawa, Yoshitsugu Shiro, Yasuhisa Mizutani, Shigetoshi Aono, 松田厚志]
通讯作者: 松田厚志
超分解能蛍光顕微鏡により観察する分裂酵母のクロマチン
使用超分辨率荧光显微镜观察裂殖酵母染色质
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [M. Tominaga, A. Kimura, E. Yokota, T. Hamaguchi, T. Shimmen, K. Yamamoto, A. Nakano, and K. Ito, H. A. Sakaue, 松田厚志]
通讯作者: 松田厚志
17
    Super-resolution, multicolor imaging of chromatin structures in the interphase nucleus
    Experimental Investigation of the Baroclinic Effect due to the Interaction between the Shock Wave and the Discharged Plasma
    • 批准号:
      23760772
    • 项目类别:
      Grant-in-Aid for Young Scientists (B)
    • 资助金额:
      $2.91万
    • 财政年份:
      2011
    • 负责人:
      MATSUDA Atsushi
    • 依托单位:
    海外基金