The response of a bony single cell to the quantitive mechanical stress by an atomic force microscope

我们检查了悬臂梁尖端的形状、材料的质量和电池的加工条件，以建立一个使用原子力显微镜对单个电池进行定量机械应力加载的系统。然后，检测加载到细胞上的刺激量。对细胞施加60nN的机械应力，钙反应分析后用光学显微镜观察，可见大量破坏细胞膜的细胞。然后，确定适合于钙反应观察并且不易对细胞膜造成损伤的刺激量。虽然测量数据应该根据这些条件进行汇总，但AFM设备因地震灾害而损坏，无法进行测量。现在，它正在为重新运行AFM设备做准备。

We examined the form at the tip of cantilever, the quality of the material, and the processing conditions of the cell towards the establishment of a system which performs quantitive mechanical stress load to a single cell using an atomic force microscope. Then, quantity of the stimulus loaded on the cell was examined. When mechanical stress was loaded on the cell by 60nN and the cell was observed with the optical microscope after calcium response analysis, many cells which damaged the cell membrane were seen. Then, the quantity of the stimulus in which it is suitable for calcium response observation, and damage to a cell membrane cannot break out easily was determined. Although measurement data was due to be totaled based on these conditions, AFM equipment is damaged for an earthquake disaster and measurement is impossible. Now, it is preparing towards re-operation of AFM equipment.