Prediction of lung cancer prognosis, and anticancer drug sensitivity by diversified clinical-gene set screening through microarray analysis, mutation, and aberrant methylation
通过微阵列分析、突变和异常甲基化进行多样化临床基因组筛选,预测肺癌预后和抗癌药物敏感性
基本信息
- 批准号:18591540
- 负责人:
- 金额:$ 2.49万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2006
- 资助国家:日本
- 起止时间:2006 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This study was performed for the purpose of clarifying prognostic factor and chemosensitivity in the lung cancer by gene expression profile analysis, aberrant methylation analysis, and mutation analysis. At first we performed gene expression profiling of 238 resected lung cancer cases using cDNA chip consisting of 11,000 genes which was developed originally in Chiba cancer center. Provided data was used as various genetic screening. Next we examined the following using the samples of 238 resected lung cancer cases in Chiba University Hospital. Methylation analyses were performed for IL-12Rβ2 gene which is one of subunit of the Interleukin-12 receptor, CXCL12 gene which is important for the metastasis formation, and Wnt antagonist genes (sFRP-1, sFRP-2, sFRP-5, Wif-1, Dkk-3) that are important for proliferation and growth. In addition, protein expression of both CXCR4 and β-catenin were examined. Finally, mutation analyses of EGFR and KRAS genes were performed. As a result, aberrant methylation of IL-12Rβ2 gene was common in lung cancer, and was a prognostic factor in adenocarcinomas. Aberrant methylation of CXCL12 was also common and was a prognostic factor in NSCLC. CXCL12 methylation as well as CXCR4 overexpression in tumor cells of primary site was considered to be important for the metastasis formation and the analysis of CXCL12 may become a useful prognostic marker. In addition, dysregulation of both Wnt and EGFR signaling pathways were common in NSCLC, and cross-talk between Wnt and EGFR has been identified in this study. The results may help foster development of chemotherapeutic treatments in NSCLC.
本研究旨在通过基因表达谱分析、异常甲基化分析和突变分析来阐明肺癌的预后因素和化疗敏感性。首先,我们利用千叶癌症中心研发的包含11,000个基因的基因芯片,对238例肺癌切除病例进行了基因表达谱分析。提供的数据用于各种遗传筛查。接下来,我们使用千叶大学医院238例肺癌切除病例的样本进行了以下检查。对IL-12受体亚基之一的IL-12Rβ-2基因、与肿瘤转移形成有关的CXCL12基因和对增殖和生长有重要作用的Wnt拮抗剂基因(SFRP-1、SFRP-2、SFRP-5、WiF-1、DKK-3)进行甲基化分析。此外,还检测了CXCR4和β-连环蛋白的蛋白表达。最后对EGFR和KRAS基因进行突变分析。因此,IL-12Rβ-2基因异常甲基化在肺癌中十分常见,是腺癌的预后因素。CXCL12基因异常甲基化也很常见,是非小细胞肺癌的预后因素。CXCL12甲基化和CXCR4在原发部位肿瘤细胞中的过度表达被认为是肿瘤转移形成的重要因素,CXCL12的分析可能成为有用的预后指标。此外,Wnt和EGFR信号通路的失调在非小细胞肺癌中很常见,在本研究中发现了Wnt和EGFR之间的串扰。这一结果可能有助于促进非小细胞肺癌化疗的发展。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Aberrant methylation of CXCL12 in non-small cell lung cancer is associated with unfavorable prognoslis
非小细胞肺癌中 CXCL12 的异常甲基化与不良预后相关
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:5.2
- 作者:Ishikura H;Ikeda H;Abe H;Ohkuri T;Hiraga H;Isu K;Tsukahara T;Sato N;Minami A;Nishimura T;Okubo Chigusa;Makoto Suzuki
- 通讯作者:Makoto Suzuki
肺癌の分子生物学とその臨床応用原発性非小細胞肺癌におけるWntシグナルとEGFRシグナルの同期的異常
肺癌分子生物学及其临床应用 原发性非小细胞肺癌Wnt和EGFR信号同步异常
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Matsuzaki J;Tsuii T;Zhang Y;Wakita D;Imazeki I;Sakai T;Ikeda H;Nishimura T;Okubo Chigusa;鈴木 実
- 通讯作者:鈴木 実
Synchronous alterations of Wnt and EGFR signaling pathways through aberrant methylation and mutation in non-small cell lung cancer
非小细胞肺癌中异常甲基化和突变导致 Wnt 和 EGFR 信号通路同步改变
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Suzuki;M.;Shigematsu;H.;Nakajima;T.;Kubo;R.;Motohashi;S.;Sekine;Y.;Shibuya;K.;Iizasa;T.;Hiroshima;K.;Nakatani;Y.;Gazdar;AF.;Fujisawa;T
- 通讯作者:T
肺癌の分子生物学とその臨床応用 原発性非小細胞肺癌におけるWntシグナルとEGFRシグナルの同期的異常
肺癌分子生物学及其临床应用 原发性非小细胞肺癌Wnt和EGFR信号同步异常
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Sasaki T;Ikeda;H;Sato M;Ohkuri T;Abe H;kuroki M;Onodera M;Miyamoto M;Kondo S;Nishimura;T;Morisita Y;Makoto Suzuki et al.;Ishiyama Tadashi;鈴木 実
- 通讯作者:鈴木 実
Aberrant methylation of CXCL12 in non-small cell lung cancer is associated with unfavorable prognosis
非小细胞肺癌中CXCL12的异常甲基化与不良预后相关
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:Suzuki;M.;Mohamed;S.;Nakajima;T.;Kubo;R.;Tian;L.;Fujiwara;T.;Suzuki;H.;Nagato;K.;Chiyo;M.;Motohashi;S.;Yasufuku;K.;Iyoda;A.;Yoshida;S.;Sekine;Y.;Shibuya;K.;Hiroshima;K. Nakatani,Y.;Yoshino,I.;Fujisawa,T.
- 通讯作者:Fujisawa,T.
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SUZUKI Makoto其他文献
KISHINEN: Video work of Creating Contemporary Japanese Garden
KISHINEN:创造当代日本花园的视频作品
- DOI:
- 发表时间:
2014 - 期刊:
- 影响因子:0
- 作者:
SATOW Simmon;SUZUKI Makoto - 通讯作者:
SUZUKI Makoto
Receiver Performance Evaluation and Fading Duration Analysis for Concurrent Transmission
并发传输的接收机性能评估和衰落持续时间分析
- DOI:
10.1587/transcom.2017ebp3169 - 发表时间:
2018 - 期刊:
- 影响因子:0.7
- 作者:
LIAO Chun-Hao;SUZUKI Makoto;MORIKAWA Hiroyuki - 通讯作者:
MORIKAWA Hiroyuki
Civic Japanese Gardens in Japan: Modern Japanese Gardens Created by the Japanese Public Sector
日本的市民日本花园:日本公共部门创建的现代日本花园
- DOI:
- 发表时间:
2014 - 期刊:
- 影响因子:0
- 作者:
MAKITA Naoko;TAKAHASHI Yoshihiro;SUZUKI Makoto - 通讯作者:
SUZUKI Makoto
奄美大島におけるリュウキュウコノハズクの繁殖成功の空間パターンと景観要因の関係
奄美大岛上琉球角鸮繁殖成功的空间格局与景观因子的关系
- DOI:
- 发表时间:
2018 - 期刊:
- 影响因子:0
- 作者:
MAKITA Naoko;TAKAHASHI Yoshihiro;SUZUKI Makoto;井上遠・松本麻依・吉田 丈人・鷲谷いづみ - 通讯作者:
井上遠・松本麻依・吉田 丈人・鷲谷いづみ
Cultural Crossing: Building the International Institute for Japanese Garden Arts and Culture
文化跨越:建设国际日本园林艺术文化学院
- DOI:
- 发表时间:
2014 - 期刊:
- 影响因子:0
- 作者:
BLOOM Stephen;DURSTON Diane;UCHIYAMA Sadafumi;SUZUKI Makoto - 通讯作者:
SUZUKI Makoto
SUZUKI Makoto的其他文献
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{{ truncateString('SUZUKI Makoto', 18)}}的其他基金
証明指導の改善に関する研究ー方針を立てることや読むことを通してー
关于改进证明教学的研究 - 通过政策制定和阅读 -
- 批准号:
20H00793 - 财政年份:2020
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Encouragement of Scientists
Novel intervention strategy for acquiring smooth behaviors: neural synchronization and behavioral learning
获得平滑行为的新颖干预策略:神经同步和行为学习
- 批准号:
18H03133 - 财政年份:2018
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
A novel intervention to modulate reciprocal inhibition function: effects of electromagnetic stimulation and afferent feedback
调节相互抑制功能的新型干预措施:电磁刺激和传入反馈的影响
- 批准号:
18K19750 - 财政年份:2018
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Challenging Research (Exploratory)
A deplopmental study on cultural instituions
文化机构的发展研究
- 批准号:
15K01149 - 财政年份:2015
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Research of Universal Management in Public Japanese Gardens in the Modern World
现代世界日本公共花园普遍管理研究
- 批准号:
26292187 - 财政年份:2014
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Research in the Reforms of the Eight Banners during the Reign of Emperor Yongzheng of the Qing Dynasty and the Appointment of Bannermen Bureaucrats
清雍正年间八旗改革及旗人任用研究
- 批准号:
25370846 - 财政年份:2013
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Paired associative stimulation for smooth movement: change in reciprocal inhibition
配对联想刺激实现平稳运动:相互抑制的变化
- 批准号:
23700633 - 财政年份:2011
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
The new approach method for the vascularization mechanism elucidation of the non-small-cell lung cancer and the clinical application
非小细胞肺癌血管化机制阐明的新方法及临床应用
- 批准号:
23592069 - 财政年份:2011
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Second messenger systems required for epithelial morphogenesis in vivo
体内上皮形态发生所需的第二信使系统
- 批准号:
23770261 - 财政年份:2011
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
The response of a bony single cell to the quantitive mechanical stress by an atomic force microscope
原子力显微镜观察骨单细胞对定量机械应力的响应
- 批准号:
22890022 - 财政年份:2010
- 资助金额:
$ 2.49万 - 项目类别:
Grant-in-Aid for Research Activity Start-up
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