The role of acetylation of histone variant H2AZ in chromatin dynamics upon DNA damage

点击翻译按钮获取中文摘要

To understand the role of acetylation in chromatin dynamics upon DNA damage, we purified the DNA helicase TAP54, which is the component of the TIP60 histone acetyltransferase (HAT) complex, from nuclear extract of HeLa cells. We found that TAP54 complex has HAT activity. Mass spetrometry (MS) analysis indicated that histone acetyltranferase, which is different from TIP60, and histone variant H2AZ were identified in the TAP54 complex. Since we have already shown that TIP60 acetylates histone variant H2AX upon DNA damage, these findings suggest that the cross talk between acetylation of H2AX and H2AZ has important roles in DNA damage response.