Molecular mechanisms underlying the kinetochore localization of Bub1

Bub1是一种保守的激酶，在忠实的染色体分离中起作用。在m期，Bub1定位于着丝点，这种着丝点定位对其功能至关重要。然而，Bub1的着丝点定位机制在很大程度上仍然未知。在这项研究中，我们发现了另一种保守的激酶Mps1磷酸化着丝点蛋白Spc7。这种磷酸化促进了Bub1和Spc7之间的相互作用，导致Bub1的着丝点定位。

Bub1 is a conserved kinase which has roles in faithful chromosome segregation. Bub1 localizes at kinetochores in M-phase and this kinetochore localization is essential for its function. However the mechanisms underlying the kinetochore localization of Bub1 remained largely unknown. In this study, we showed that another conserved kinase Mps1 phosphorylates the kinetochore protein Spc7. This phosphorylation promotes the interaction between Bub1 and Spc7, resulting in the kinetochore localization of Bub1.