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ANALYSIS OF GENOMIC STRUCTURE OF HEPATITIS G VIRUS (GBV-C/HGV) AND DEVELOPMENT OF SENSITIVE DETECTION SYSTEM OF THE VIRAL GENOME

庚型肝炎病毒(GBV-C/HGV)基因组结构分析及病毒基因组灵敏检测系统的开发

基本信息

批准号：
09470087
负责人：
OKAMOTO Hiroaki
金额：
$ 6.66万
依托单位：
JICHI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 2000
项目状态：
已结题

项目摘要

During 1995 and 1996, a possible agent for non-A to E hepatitis, designated hepatitis G virus (HGV) or GB virus C (GBV-C), was discovered in the United States. In this research project, the entire nucleotide sequence of Asian HGV strain (GT230 [9390 nucleotides]) was determined and its genomic organization was characterized. The GT230 isolate lacked a clearly identifiable core gene as in the reported isolates. The 3'-untranslated region was highly conserved and utilized as a target for the sensitive detection of HGV RNA of all five genotypes (G1-G5) by the RT-PCR.HGV RNA was detected in 15 (1.2%) of the 1303 healthy blood donors. Of the 2979 women, 32 (1.1%) were positive for HGV RNA.Twenty-six (77%) of 34 babies born to these viremic women were positive for the virus, indicating that HGV is frequently transmitted from mother to infant in the general population. The most critical factor was the HGV viral load in the maternal serum. The genotype G3 was prevalent in healthy individuals and hepatitis patients. Whereas other genotypes of HGV were frequently found in patients with hemophilia who had received imported coagulation factor concentrates in the past.The HGV prevalence in patients with non-B non-C chronic liver disease was comparable with that in patients with type B or type C chronic liver disease, and no difference was observed in demographic features, biochemical factors and histological findings among patients with or without HGV, suggesting that the contribution of HGV infection to liver diseases of unknown etiology would be small. However, certain HGV strain (s) or emerging variant (s) may be pathogenic under special clinical and epidemiological settings. We must keep HGV under suspicion and under study. More evidence is needed before we can reach a final decision about the pathogenesis of HGV.

1995-1996年间，在美国发现了一种可能的非甲至戊型肝炎的病原体，命名为庚型肝炎病毒(HGV)或丙型肝炎病毒(GBV-C)。本研究测定了亚洲HGV株(GT230[9390个核苷酸])的全序列，并对其基因组结构进行了分析。与已报道的分离株不同，GT230分离株缺乏清晰可识别的核心基因。在1303例健康献血员中，有15例(1.2%)检出HGV RNA，其中15例检出HGV RNA。在2979名妇女中，32名(1.1%)HGV RNA阳性，这些病毒携带者所生的34名婴儿中有26名(77%)HGV阳性，这表明HGV在普通人群中经常通过母亲传染给婴儿。最关键的因素是母亲血清中的HGV病毒载量。G3基因在健康人和肝炎患者中普遍存在。非乙非丙型慢性肝病患者的HGV感染率与B型或C型慢性肝病患者的HGV感染率相当，HGV携带者和无HGV携带者的人口学特征、生化指标和组织学检查结果无差异，提示HGV感染对不明原因肝病的贡献较小。然而，在特殊的临床和流行病学环境下，某些庚型肝炎病毒株(S)或新出现的变异株(S)可能是致病的。我们必须保持对HGV的怀疑和研究。在我们对HGV的发病机制做出最终决定之前，还需要更多的证据。