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Development of genotyping method for TT virus (TTV) and its application to elucidation of pathogenesis.

TT病毒（TTV）基因分型方法的开发及其在阐明发病机制中的应用。

基本信息

批准号：
13357003
负责人：
OKAMOTO Hiroaki
金额：
$ 17.22万
依托单位：
JICHI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13357003/
关键词：
TTV Tprque genotype virus phylogenetic tree PCR Genetic group selective PCR selective PCR TTウイルス(TTV)Neighbor-joining法 TTウイルス

项目摘要

In 1997, we isolated a novel DNA virus from the serum of a patient with posttransfusion acute hepatitis of unknown etiology and named it TT virus (TTV) after the initials (T.T.) of the index patient. Recently, taxonomic nomenclature for TTV has been proposed by the TTV study group of the International Committee on Taxonomy of Viruses (ICTV) : head investigator of this project (H.O.) was a member of the study group. It was designated as Torque Teno virus (TTV): this name was derived from "Torque" which means the necklace, and "Tenuis" means thin, and relates to circular, single-stranded nature of its DNA genome. TTV was classified into a novel floating genus, the Anellovirus genus, which has not yet been assigned to a family. The name of the genus, Anellovirus, was derived from "anello," which means the ring, and relates to the circular nature of the DNA genome. TTV has a wide range of sequence divergence for a DNA virus, and approximately 40 genotypes have been identified. In the present study, we classified them further into five distantly related genetic groups (Groups 1-5), and developed a selective PCR method using oligonulceotide primers specific for TTVs of each of the five genetic groups to classify TTVs into the five genetic groups. Using this PCR method, we examined the prevalence of five genetic groups of TTV in relation to age. TTVs of group 4 were prevalent in early childhood, and detected most frequently in adulthood among the five known genetic groups. TTVs of group 1were detectable in infants aged> 6 months. Its frequency increased with age and was 10-15% in adulthood. TTVs of group 3 comprised the greatest number of genotypes (15 genotypes including SENV of genotypes A to H), were coinfected with TTVs of group 1 and/or TTVs of group 4 in adulthood. Presently, we have established a real-time detection method for quantitating TTVs of group 1,2,3,4 or 5 with an attempt to apply it to clinical surveys.

1997年，我们从一例原因不明的输血后急性肝炎患者的血清中分离到一种新的DNA病毒，并以首字母(T.T.)命名为TT病毒(TTV)。指标患者的。最近，国际病毒分类委员会(ICTV)的TTV研究小组提出了TTV的分类命名：该项目的首席研究员(H.O.)是学习小组的一员。它被命名为Torque Teno病毒(TTV)：这个名字源于TORQUE，意思是项链，Tenuis意味着瘦，与其DNA基因组的环状、单链性质有关。TTV被归入一个新的漂浮属，即AnelloVirus属，该属尚未被归入一个科。该属的名称AnelloVirus源自Anello，意思是环，与DNA基因组的环状性质有关。TTV对一种DNA病毒具有广泛的序列差异，目前已鉴定出约40种基因类型。在本研究中，我们将它们进一步划分为五个远缘关系基因组(组1-5)，并利用针对这五个基因组中每一个TTV的特异性寡核苷酸引物建立了选择性PCR方法，将TTV分为五个基因组。使用这种聚合酶链式反应方法，我们检查了与年龄相关的TTV的五个基因组的患病率。在已知的5个遗传组中，第4组TTV在儿童早期流行，且在成年期最常见。第1组TTV在6个月龄婴儿中均可检出。其发生频率随年龄增长而增加，成年期为10-15%。第3组TTV的基因分型最多(包括A~H型SENV在内的15种基因型)，成年期与第1组TTV和/或第4组TTV混合感染。目前，我们已经建立了1、2、3、4、5组TTV的实时检测方法，并试图将其应用于临床调查。