Application Studies on Product-Immobilization Phenomenon Newly Discovered
新发现的产品固定现象的应用研究
基本信息
- 批准号:10556027
- 负责人:
- 金额:$ 4.99万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B).
- 财政年份:1998
- 资助国家:日本
- 起止时间:1998 至 2000
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Recently we have found the "immobilization of product" in the enzyme-digestion of starch granule, in which the product precipitates with granule. The phenomenon is of importance in i) the easy recovery of product under the less energy and ii) the inclusion of effective compounds into the polysaccharide. In this project, the fundamental study was done to learn the phenomenon, and the application of unutilized or low-utilized polysaccharides to the food industry was tried to be established. The results obtained were as follows.(1) Cellulose. i) The product-immobilization was also found in the degradation of cellulose by cellulase. ii) Products in the cellulose of precipitate were water-soluble oligosaccharides of short-chain. iii) The inside product increased by addition of ethanol to reaction mixture. Saccharides in the cellulose was easily recovered under the low concentration of ethanol. iv) Production of oligosaccharides was done using ethanol. After enzyme reaction the supernatant w … More as discarded, and then water was added to the precipitate, releasing oligosaccharides of different size. Isolation was done by gel-filtration and HPLC.(2) Chitin and xylan. i) In the enzymatic degradation, the product-immobilization was also found. However, the included amount was small as compared with starch granule and cellulose. ii) The addition of ethanol increased products in the two polysaccharides. (3) Porous starch granule as inclusion material. i) Porous starch granules of various plant origins were prepared by enzyme treatment. There were two types of starches which formed pores or no pore on the surface of granule. ii) The pore size could be controlled by amount and treatment time of enzyme. iii) The smallporous granule was able to incorporate the short-chain oligosaccharides, and the long-chain one entered the starch of large pore. iv) Ascorbic acid was also incorporated into the porous starch granule. After the granule including ascorbic acid was transferred into water, the compound gradually released into water. Less
近年来,在淀粉颗粒的酶解过程中发现了“产物固定化”现象,即产物随颗粒沉淀。该现象在以下方面是重要的:i)在较少的能量下容易地回收产物和ii)将有效化合物包含到多糖中。本课题对这一现象进行了基础性研究,并试图建立未利用或利用率低的多糖在食品工业中的应用。获得的结果如下。(1)纤维素.(1)在纤维素酶降解纤维素的过程中也发现了固定化产物的现象。ii)纤维素沉淀产物为短链水溶性低聚糖。iii)通过向反应混合物中加入乙醇来增加内部产物。在低浓度乙醇条件下,纤维素中的糖苷易于回收。iv)使用乙醇进行寡糖的生产。酶反应后的上清液w ...更多信息 弃去,然后向沉淀中加入水,释放出不同大小的寡糖。通过凝胶过滤和HPLC进行分离。(2)甲壳素和木聚糖。(1)在酶解过程中,也发现了产物的固定化,但固定化产物的量与淀粉颗粒和纤维素相比较少。ii)乙醇的加入增加了两种多糖中的产物。(3)包合材料为多孔淀粉颗粒。i)通过酶处理制备各种植物来源的多孔淀粉颗粒。淀粉颗粒表面有气孔和无气孔两种类型。ii)孔径大小可以通过酶的用量和处理时间来控制。(3)小孔颗粒能够吸收短链寡糖,长链寡糖进入大孔淀粉中。iv)还将抗坏血酸掺入多孔淀粉颗粒中。将含有抗坏血酸的颗粒转移到水中后,化合物逐渐释放到水中。少
项目成果
期刊论文数量(102)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Y.Nagasaka: "Studies on Glucomylase from Corticium rolfsii"J.Appl.Glycosci.. 46・2. 169-178 (1999)
Y.Nagasaka:“来自 Corticium rolfsii 的葡萄糖化酶的研究”J.Appl.Glycosci.. 46・2(1999)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Y.Nagasaka, N.Muraki, A.Kimura, K.Kitamoto, M.Sotou, A.Yokota & F.Tomita: "Studies on Glucomylase from Corticium rolfsii"J.Appl.Glycosci.. 46-2. 169-178 (1999)
Y.Nagasaka、N.Muraki、A.Kimura、K.Kitamoto、M.Sotou、A.Yokota
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
A.Kimura: "Immobilization of Product Observed in Digestion of Soluble Starch with Enzyme"Brain Techno News. 72・3. 27-29 (1999)
A.Kimura:“用酶消化可溶性淀粉时观察到的产物的固定化”Brain Techno News 72・3(1999)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
T.Mizuno: "Molecular Cloning of Isomltotrio-Dextranase Gene from Brevibacterium fuscum var. dextranlyticum Strain 0407 and Its Expression in Esherichia coli"Biosci.Biotechnol.Biochem.. 63・9. 1582-1588 (1999)
T. Mizuno:“来自短杆菌右旋糖酐变种菌株 0407 的异三葡聚糖酶基因的分子克隆及其在大肠杆菌中的表达” Biosci.Biotechnol.Biochem.. 63・9 (1999)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
S.-M.Jun: "The Effects on Escherichia coli and Plasmid DNA Using Ultrasoft X-ray"Kor.J.Biotechnol.Bioeng.. 15・1. 84-87 (2000)
S.-M.Jun:“使用超软X射线对大肠杆菌和质粒DNA的影响”Kor.J.Biotechnol.Bioeng.. 15・1(2000)
- DOI:
- 发表时间:
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- 影响因子:0
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KIMURA Atsuo其他文献
KIMURA Atsuo的其他文献
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{{ truncateString('KIMURA Atsuo', 18)}}的其他基金
Problem-based researches utilized by novel megalosaccharides dissolving poorly-soluble BCS II compounds
利用新型巨糖溶解难溶性 BCS II 化合物的基于问题的研究
- 批准号:
19KK0147 - 财政年份:2019
- 资助金额:
$ 4.99万 - 项目类别:
Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
Further development of megalosaccharide research: synthesis and application of novel megalosaccharides displaying excellent functions
巨糖研究进一步发展:功能优异的新型巨糖的合成及应用
- 批准号:
17H03801 - 财政年份:2017
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Novel mechanism of starch-granule degradation and its application
淀粉颗粒降解新机制及其应用
- 批准号:
26660079 - 财政年份:2014
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Construction of novel molecular mechanism on polysaccharide-forming enzyme and its application
多糖形成酶新分子机制的构建及其应用
- 批准号:
24658089 - 财政年份:2012
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Establishment of novel reaction mechanism on glycosylase and its application
糖基酶新反应机制的建立及其应用
- 批准号:
22658032 - 财政年份:2010
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Survey on members of sucrose-world in Asian tropical reef, namely in Thailand
亚洲热带珊瑚礁即泰国蔗糖世界成员调查
- 批准号:
21405020 - 财政年份:2009
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of structural elements controlling transglycosylation and its application
控制转糖基化的结构元件分析及其应用
- 批准号:
20380056 - 财政年份:2008
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Investigation on Molecular Mechanism of Sucrose-degrading Enzyme in Asian Honeybee.
亚洲蜜蜂蔗糖降解酶分子机制的研究。
- 批准号:
16405025 - 财政年份:2004
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Research on Glycosylases which Obtained New Functions by Mutation on Catalytic Residue.
通过催化残基突变获得新功能的糖基化酶的研究。
- 批准号:
14360043 - 财政年份:2002
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular Mechanism of Novel N-Linked Sugar Chain Having α-Galactofuranosyl Structure
具有α-呋喃半乳糖基结构的新型N-连接糖链的分子机制
- 批准号:
10660068 - 财政年份:1998
- 资助金额:
$ 4.99万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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