Establishment of novel reaction mechanism on glycosylase and its application

提出了两种反应机制：在糖基酶上的反应机制，即氧羰基离子介导的反应机制和共价键的形成机制。最近，后者得到了许多糖基酶科学家的支持。然而，后一种机制存在一个问题：这种机制无法得到有机化学相关知识的支持。本项目的目的是对氧-碳离子介导的机理进行实验证明。糖基化酶催化有用的转糖基化反应。这一反应机理可能产生新的应用途径，并对其可能性进行了探讨。

Two reaction mechanisms have been proposed : on glycosylases, i. e. oxo-carbenium ion-mediated mechanism and covalent linkage-formation mechanism. Recently, the latter one is supported by many glycosylase-scientists. However, there is a problem in the latter mechanism : this mechanism cannot be supported by organic chemistry-related knowledge. A purpose of this project is an experimental proof of the oxo-carbenium ion-mediated mechanism. Glycosylases catalyze the useful reaction of transglycosylation. This reaction mechanism may produce the novel application approach, the possibility of which is also investigated.