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Investigation on Molecular Mechanism of Sucrose-degrading Enzyme in Asian Honeybee.

亚洲蜜蜂蔗糖降解酶分子机制的研究。

基本信息

批准号：
16405025
负责人：
KIMURA Atsuo
金额：
$ 5.31万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16405025/
关键词：
Asian honeybee α-glucosidase honey 国際情報交換タイ:韓国

项目摘要

We have analyzed three sucrose-degrading isozymes (α-glucosidase I,II, and III) in European honeybees, since these enzymes exhibited the interesting activity to sucrose of high concentration. α-Glucosidase I was a unique allosteric enzyme to display negative cooperativity in extremely high sucrose concentration. α-Glucosidase II also exhibited the allosteric property of positive cooperativity. α-Glucosidase III was a Michaelis-Menten-type enzyme to be secreted to nectar (bees gathered from flower) and to contribute to the formation of honey. Purpose of this project is i)existence of allosteric α-glucosidase in Asian honeybees, ii)investigation of honey-forming mechanism, and iii)regulation in expression of three α-glucosidase isozymes.Four kinds of honeybee species were investigated in this research. From typical Asian honeybees (living in Thailand, Korea and Japan), three isozymes were isolated by chromatographic method. One of them was an allosteric enzyme having similar properties t … More o European honeybee α-glucosidase I, allowing to molecular analysis of negative cooperativity. The second enzyme was α-glucosidase II-type isozyme. Enzyme in honey was also investigated, and showed similar to one of enzymes (α-glucosidase III-type isozyme). cDNAs of these three enzyme were cloned. It was found that the expression of three isozymes was regulated. In small honeybees living in Thailand, three α-glucosidases were also found, in which two α-glucosidases were isolated. Their properties investigated were almost identical to those of α-glucosidases I and II. Enzyme in honey was compared with adult bee enzymes. All honeybee species studied in this project contained allosteric enzyme. Asian honeybees were divided into two types : i)having three α-glucosidase isozymes and ii)having two α-glucosidase isozymes. Recently, we found that bumblebees also had two α-glucosidase isozymes. Further research will elucidate the interesting molecular mechanism of sucrose-degrading enzymes in flower bees. Less

我们分析了欧洲蜜蜂中的三种蔗糖降解同工酶(α-葡萄糖苷酶I、II和III)，因为这些酶对高浓度的蔗糖表现出有趣的活性。α-葡萄糖苷酶I是唯一一种在极高蔗糖浓度下表现负协同作用的变构酶。α-葡萄糖苷酶II也表现出正协同的变构性质。α-葡萄糖苷酶III是一种米氏类型的酶，分泌到花蜜(蜜蜂从花中采集)，并有助于形成蜂蜜。本研究的目的是：1)亚洲蜜蜂体内存在变构α-葡萄糖苷酶，2)研究成蜜机理，3)3种α-葡萄糖苷酶同工酶的表达调控。从典型的亚洲蜜蜂(生活在泰国、韩国和日本)中，用层析方法分离出三种同工酶。其中之一是一种变构酶，其性质与…相似更多的欧洲蜜蜂α-葡萄糖苷酶I，允许对负协同性进行分子分析。第二种酶为α-葡萄糖苷酶II型同工酶。对蜂蜜中的酶也进行了研究，发现其类似于一种酶(α-葡萄糖苷酶III型同工酶)。克隆了这三种酶的cDNA。结果表明，三种同工酶的表达均受调控。在生活在泰国的小蜜蜂中，也发现了三种α-葡萄糖苷酶，其中两种α-葡萄糖苷酶被分离出来。它们的性质与α-葡萄糖苷酶I和II几乎相同。蜂蜜中的酶与成蜂的酶进行了比较。本项目所研究的所有蜜蜂物种都含有变构酶。亚洲蜜蜂分为两种类型：1)有3个α-葡萄糖苷酶同工酶；2)有2个α-葡萄糖苷酶同工酶。最近，我们发现大黄蜂也有两种α-葡萄糖苷酶同工酶。进一步的研究将阐明花蜂蔗糖降解酶有趣的分子机制。较少