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METHOD FOR LONG-TERM MEASUREMENTS OF SYMPATHETIC NERVE ACTIVITY IN FREELY MOVING RATS.

长期测量自由活动大鼠交感神经活动的方法。

基本信息

批准号：
10557007
负责人：
MIKI Kenju
金额：
$ 7.36万
依托单位：
Nara Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10557007/
关键词：
Sympatheticnerve activity Exercise Rat Cardiovascular function

项目摘要

The sympathetic nervous system is believed to play a major role in regulating cardiovascular function during exercise. However, only a few direct measurements of sympathetic nervous activity during whole body dynamic exercise have been attempted.In the present study, we have established methods for the measurements of the renal sympathetic nerve activity (RSNA) and cardiovascular function during treadmill exercise in rats. We trained Wistar rats to run on the treadmill over a week before the surgery. At least 2 days before the experiment, electrodes for the measurements of RSNA and electrocardiogramand electromyogram, catheters for the measurements of systemic arterial and central venous pressures were implanted under aseptic condition.The responses in RSNA to treadmill exercise and spontaneous movement was measured with a satisfactory signal to noise ratio for over a week. We observed no contamination by noise signals, which could have originated from the EMG and ECG or the experimental equipment, including the treadmill, on the RSNA recorded during the tread-mill exercise and voluntary movement. The ratio of RSNA signal to noise was enough to evaluate the response of sympathetic nerve activity to voluntary and involuntary movements in rats. This level of RSNA signal with satisfactory signal to noise ratio, lasted over a week after the surgery in approximately 70% of the animals of fifty rats. This experimental model allows us to study the neural mechanisms involved in the regulation of cardiovascular function during dynamic exercise in rats.

交感神经系统被认为在运动期间调节心血管功能中起主要作用。然而，只有少数人尝试在全身动力运动中直接测量交感神经活动，在本研究中，我们建立了大鼠在跑台运动中肾交感神经活动（RSNA）和心血管功能的测量方法。我们在手术前一周训练Wistar大鼠在跑步机上跑步。实验前至少2天，在无菌条件下植入RSNA、心电图和肌电图测量电极，体动脉压和中心静脉压测量导管，连续1周以上测量RSNA对跑台运动和自发运动的反应，信噪比良好。我们没有观察到噪声信号的污染，这可能是源于肌电图和心电图或实验设备，包括跑步机，在跑步机运动和自主运动期间记录的RSNA。RSNA信噪比足以评价大鼠交感神经活动对随意运动和不随意运动的反应。手术后，在50只大鼠的约70%的动物中，具有令人满意的信噪比的RSNA信号水平持续了一周以上。该实验模型使我们能够研究大鼠在动态运动过程中参与心血管功能调节的神经机制。