Experimental model for studying health promotion and preventive medicine using rats.

使用大鼠研究健康促进和预防医学的实验模型。

• 批准号: 07558136
• 负责人: MIKI Kenju
• 金额: $ 1.28万
• 依托单位: Nara Women's University, Life Science & Human Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558136/
• 关键词: rats; life style; cadiovascular function; environment

Life style is a major factor influencing health status.causing. To prevent diseases such asobesity, hypertension, and diabetes, proper way of living including meal, environmental temperature, resting manner should be suggested by medical professional. However, little information is available on this issue at present.The purpose of the present study is to establish a experimental model for studying the proper life style to prevent disease and to promote the health. Wistar rats was anesthetized with pentobarbital sodium (50 mg/kg ip), and arterial and venous catheters were inserted into the right femoral artery and vein, respectively. Then, the left kidney was also exposed retro-peritoneally and with the aid of an operation microscope, a branch of the sympathetic nerves to the kidney was dissected free and placed on a fine stainless steal bipolar electrode A thermocouple (copper-constantan) was placed in the intra-abdominal space for the measurement of core body temperature. The electrodes for the measurements of electroencephalogram and electromusculargram were also implanted. At least 2 days after the recovery period, experiment started.This methods allows us to study the effect of the changes in life style including meal, environmental temperature, sleep time, on the cardiovascular, autonomic and energy metabolism functions.

生活方式是影响健康状况的主要因素。为了预防肥胖、高血压、糖尿病等疾病，医务人员应建议适当的生活方式，包括饮食、环境温度、休息方式。本研究的目的是建立一个实验模型，为研究预防疾病、促进健康的适宜生活方式提供实验依据。Wistar大鼠用戊巴比妥钠(50 mg/kg，ip)麻醉，分别于股动脉和股静脉插管。然后将左肾暴露于腹膜后，在手术显微镜下游离肾交感神经分支，置于不锈钢双极电极上，在腹内间隙放置热电偶(铜-康铜)测量核心体温。植入用于脑电和肌电测量的电极。恢复期后至少2天开始实验，研究饮食、环境温度、睡眠时间等生活方式的变化对心血管、自主神经和能量代谢功能的影响。

项目成果

Wada, F., S. Sagawa, K. Miki, K. Nagaya, S. et al.: "Mechanism of thirst attenuation druing head-out water immersion in men." Am. J. Physiol. : Regulatory Integrative Comp. Physiol.268. R538-R589 (1995)

Wada, F., S. Sakawa, K. Miki, K. Nagaya, S. 等人：“男性头部浸水导致口渴减弱的机制。”

Shiraki, K., Sagawa S., Miki K., and Tajima F.: "Responses of muscle sympathetic nerve activity to a transient raise in core temperature in humans." Body Temperature and Metabolism, edited by Nagasaka T.and Milton A.S., IPEC press. 133-136 (1995)

Shiraki, K.、Sakawa S.、Miki K. 和 Tajima F.：“肌肉交感神经活动对人类核心温度短暂升高的反应。”

Miki K: "Effects of 2-deoxy-D-qlucose on energy metabolism and renal sympathetic nerve activity in conscious rats." Body Temperature and Metabolism. IPEC press. 28-30 (1995)

Miki K：“2-脱氧-D-葡萄糖对清醒大鼠能量代谢和肾交感神经活动的影响。”

三木健寿、能勢博: "運動と体液(第4章)、最新運動生理学" 宮村実晴編集、真興交易医書出版部, 273-296 (1996)

三木贤二、野濑宏：《运动与体液（第4章）最新运动生理学》宫村美晴主编，新光贸易医学出版部，273-296（1996）

Wada, F., S.Sagawa, K.Miki, K.Nagaya, S.Nakamitsu, K.Shiraki, and J.E.Greenleaf: "Mechanism of thirst attenuation during head-out water immersion in men." Am.J.Physiol. Regulatory Integrative Comp.Physiol.268. R538-R589 (1995)

Wada, F.、S.Sakawa、K.Miki、K.Nagaya、S.Nakamitsu、K.Shiraki 和 J.E.Greenleaf：“男性头出水浸时口渴减弱的机制。”