Search for biological active molecules from tick salivary glands

从蜱唾液腺中寻找生物活性分子

• 批准号: 15380206
• 负责人: ONUMA Misao
• 金额: $ 9.73万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15380206/
• 关键词: anti-tick vaccine; tick saliva proteins; anti-coagulant; Haemaphysalis tick; Immunosuppression; ダニ; Haemaphysalis longicornis; 遺伝子解析

To obtain biological active molecules from tick, a cDNA library was constructed from semi-fed female Haemaphysalis longicornis salivary glands. Randomly selected clones were sequenced and total of 653 sequences were analyzed by bioinformatic programs including NCBI blastX. The sequences were assembled into 321 non-redundant clusters. About 35 % of the sequences showed significant similarity to known genes in homology search against non-redundant protein database and suggested as function predictable genes whereas rest of the 65 % had no homologs. Two thirds of the function predictable sequences were suggested to be so-called house keeping genes. Among non-house keeping genes, function annotated genes which we found were as follows ; several protease inhibitors, two metalloproteases, anti-thrombin type coagulation inhibitors previously identified from H. longicpornis and a homolog of D.andersoni derived predicted immunosuppressant protein (Da-p36). Recombinant of homolog protein of Da-p36 showed an immunosuppressive activity against bovine lymphocytes. These proteins may play important roles during tick feeding and are expected to be novel tick vaccine antigen candidates.

为了从蜱类中获得生物活性分子，构建了长角血蜱（Haemaphysalis longicornis）半饥饿雌性唾液腺cDNA文库。随机选择克隆测序，并通过包括NCBI blastX的生物信息学程序分析总共653个序列。将序列组装成321个非冗余簇。在非冗余蛋白质数据库中同源性搜索发现，约35%的序列与已知基因有显著的相似性，提示为功能可预测基因，其余65%的序列无同源性。三分之二的功能可预测序列被认为是所谓的管家基因。在非管家基因中，我们发现的功能注释基因有：几个蛋白酶抑制剂，两个金属蛋白酶，抗凝血酶型凝血抑制剂，以前从H. longicpornis的同源物和源自D.andersoni的预测的免疫抑制蛋白（Da-p36）。重组Da-p36同源蛋白对牛淋巴细胞具有免疫抑制活性。这些蛋白可能在蜱虫取食过程中发挥重要作用，有望成为新的蜱虫疫苗候选抗原。

项目成果

Nakajima M., et al.: "Production and characterization of monoclonal antibodies against midgnt of ixodid tick Haemaphysalis longicornis"Vet.Parasitol.. 115. 355-363 (2003)

Nakajima M.等人：“针对长角血蜱中的蜱虫的单克隆抗体的生产和表征”Vet.Parasitol.. 115. 355-363 (2003)

Passive immunization with monoclonal antibodies : effects on Haemaphysalis longicornis tick infestation of BALB/c mice.

单克隆抗体被动免疫：对 BALB/c 小鼠长角血蜱感染的影响。

• 发表时间: 2003
• 期刊: Jpn. J. Vet. Res. 50(4)
• 作者: Nakajima M.

Production and characterization of monoclonal antibodies against midgnt of ixodid tick Haemaphysalis longicornis.

针对长角血蜱中蜱的单克隆抗体的产生和表征。

• 发表时间: 2003
• 期刊: Vet. Parasitol. 115
• 作者: Nakajima M.

A serine proteinase inhibitor (serpin) from ixodid tick Haemaphysalis longicornis; cloning and preliminary assessment of its suitability as a candidate for a tick vaccine.

• DOI: 10.1016/s0264-410x(03)00167-1
• 发表时间: 2003-06
• 期刊: Vaccine
• 影响因子: 5.5
• 作者: M. Sugino;S. Imamura;A. Mulenga;M. Nakajima;A. Tsuda;K. Ohashi;M. Onuma

Cloning, expression and partial characterization of a Haemaphysalis longicornis and a Rhipicephalus appendiculatus glutathione S-transferases.

长角血蜱和附肢扇头蜱谷胱甘肽 S-转移酶的克隆、表达和部分表征。

• 发表时间: 2004
• 期刊: Insect Biochem. Mol. Biol. 13
• 作者: da Silva Vaz.