Genetic diversity of bovine lentivirus distributed in south Asian countrirs
南亚国家牛慢病毒遗传多样性
基本信息
- 批准号:15405035
- 负责人:
- 金额:$ 9.02万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
To understand the genetic diversity of bovine lentivirus (BIV), we have examined serological survey of BIV infection in cattle in Hokkaido and found 10% of positive reactors. From the seropositive animals, 3 BIV were isolated and DNA samples were obtained from peripheral blood lymphocytes of these positive animals for the detection of BIV proviral DNA by nested-PCR to detect a part of the pol gene. A 298-bp pol region fragment specific to BIV proviral DNA was amplified. Nucleotide sequences of the amplified fragments from Japanese BIV field isolates had 99% homology with American isolate, R-29. Amplification of the C2, V1, V2 and C3 regions of the env gene of Japanese BIV isolates were carried out by nested PCR. All 3 isolates seemed to be smaller, several base substitutions were observed in the V1 region and deletions were found in the V2 region of the env gene when compared to that of American R-29 isolate.In Cambodia, samples from 544 cattle and 43 water buffalo were obtained and BIV seropositive rate were 26% in cattle and 17% in water buffalo. Five BIV proviruses were obtained from seropositive animals. Nucleotide sequence of pol region was highly conserved when compared to that of American and Japanese BIV. In contrast, env region of Cambodian BIV field isolates seemed to be smaller than those of American isolates. Nucleotide substitutions which alter amino acid sequences were observed in the V2 region of Cambodian isolates.Total 262 serum samples were obtained from cattle in Zambia and seropositive rate was 11.4%. Four BIV proviruses were obtained by nested PCR. Nucleotide sequences of pol region of these proviruses were highly homologous (over 97%) to that of American and Japanese isolates.
为了了解牛慢病毒(BIV)的遗传多样性,我们对北海道牛的BIV感染进行了血清学调查,发现10%的反应阳性。从血清阳性动物中分离出3只BIV,从阳性动物的外周血淋巴细胞中提取DNA样本,采用巢式pcr检测BIV前病毒DNA,检测部分pol基因。扩增出一个298 bp的BIV前病毒DNA特异性pol区片段。日本BIV野株扩增片段的核苷酸序列与美国分离株R-29具有99%的同源性。采用巢式PCR扩增日本BIV分离株env基因的C2、V1、V2和C3区。与美国R-29分离株相比,3株分离株的env基因在V1区有几个碱基替换,在V2区有缺失。在柬埔寨,采集了544头牛和43头水牛的样本,牛和水牛的BIV血清阳性率分别为26%和17%。从血清阳性动物中获得5种乙肝病毒原病毒。与美国和日本BIV相比,pol区核苷酸序列高度保守。相比之下,柬埔寨BIV田间分离株的环境区域似乎小于美国分离株。在柬埔寨分离株的V2区观察到核苷酸取代改变氨基酸序列。在赞比亚共获得262份牛血清样本,血清阳性率为11.4%。采用巢式PCR法获得4株乙型肝炎病毒原病毒。这些原病毒的pol区核苷酸序列与美国和日本分离株高度同源(超过97%)。
项目成果
期刊论文数量(18)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Usui T., et al.: "Protective effects vaccination with bovine leukemia virus (BLV) tax DNA against BLV infection in Sheep"J.Vet.Med.Sci.. 65(11). 1201-1205 (2003)
Usui T.等人:“用牛白血病病毒(BLV)对DNA进行疫苗接种以对抗绵羊BLV感染的保护作用”J.Vet.Med.Sci. 65(11)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Evidence for the presence of bovine immunodeficiency virus in cattle in Turkey
土耳其牛体内存在牛免疫缺陷病毒的证据
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Konnai S;Onuma M;et al.;Meas S.et al.;Meas S.et al.;小沼 操;Usui T.et al.;Meas S.et al.
- 通讯作者:Meas S.et al.
Konnai S., et al.: "Sequences and diversity of 17 new Over-DRB1 alleles from three breeds of sheep"Eurp.J.Immunogenetic.. 30. 275-282 (2003)
Konnai S.等人:“来自三种绵羊品种的 17 个新 Over-DRB1 等位基因的序列和多样性”Eurp.J.Immunogenic.. 30. 275-282 (2003)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Protective effects vaccination with bovine leukemia virus (BLV) tax DNA against BLV infection in Sheep
牛白血病病毒 (BLV) 疫苗接种对绵羊 BLV 感染的保护作用
- DOI:
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Konnai S;Onuma M;et al.;Meas S.et al.;Meas S.et al.;小沼 操;Usui T.et al.
- 通讯作者:Usui T.et al.
Meas S., et al.: "Evidence for the presence of bovine immunodeficiency virus in cattle in Turkey"Jpn.J.Vet.Res.. 51(1). 3-8 (2003)
Meas S. 等人:“土耳其牛体内存在牛免疫缺陷病毒的证据”Jpn.J.Vet.Res. 51(1)。
- DOI:
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- 期刊:
- 影响因子:0
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{{ truncateString('ONUMA Misao', 18)}}的其他基金
Genetic analysis of drug resistant trypanosomes
耐药锥虫的遗传分析
- 批准号:
18405037 - 财政年份:2006
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Search for biological active molecules from tick salivary glands
从蜱唾液腺中寻找生物活性分子
- 批准号:
15380206 - 财政年份:2003
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of tick-encoded immunoregulatory serine proteinase inhibitors (serpins)
蜱编码的免疫调节丝氨酸蛋白酶抑制剂(丝氨酸蛋白酶抑制剂)的分析
- 批准号:
13460128 - 财政年份:2001
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular mechanisms of diversity of theileria parasite genes distributed in middle and South America
中南美洲泰勒虫寄生虫基因多样性的分子机制
- 批准号:
12575027 - 财政年份:2000
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Development of anti-tick vaccine
抗蜱疫苗的研制
- 批准号:
12356008 - 财政年份:2000
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular mechanisms of diversity of theileria parasite genes and control of the disease
泰勒虫寄生虫基因多样性的分子机制及其疾病控制
- 批准号:
11460141 - 财政年份:1999
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Molecular evolution of Theileria parasites in Southern Asia
南亚泰勒虫寄生虫的分子进化
- 批准号:
10041150 - 财政年份:1998
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (A).
Immunological modulation and control of infections diseases by cytokaines
细胞因子对感染性疾病的免疫调节和控制
- 批准号:
09356009 - 财政年份:1997
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular evolution of Theileria parasites in Pacific area
太平洋地区泰勒虫寄生虫的分子进化
- 批准号:
08041130 - 财政年份:1996
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for international Scientific Research
Molecular escape mechanisms of Theileria parasites
泰勒虫寄生虫的分子逃逸机制
- 批准号:
07406019 - 财政年份:1995
- 资助金额:
$ 9.02万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
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