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Molecular mechanisms of diversity of theileria parasite genes and control of the disease

泰勒虫寄生虫基因多样性的分子机制及其疾病控制

基本信息

批准号：
11460141
负责人：
ONUMA Misao
金额：
$ 9.79万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

Theileria sergenti is a tick-borne protozoa of cattle. Benign bovine theileria parasites, which are presumed to be of the T.sergenti/buffeli/orientalis group are distributed in Asian countries. The parasites have the major piroplasm surface protein (MPSP) gene which is one of the means of parasite evasion of host immunity. T.sergenti has 4 chromosomes similar to T.parva.Most of genes used for molecular karyotype showed a conserved synteny between T.sergenti and T.parva.To underatand the genome mapping of theileria parasites, characterization of bovine genes expressed in T.parva transformed T-lymphoblastoid cell lines was performed by using expressed sequence tag (EST) sequencing approach of random cDNA clones from libraries. Single pass sequencing was done on 394 cDNA clones and the sequences search for significant homolgies in GeneBank, EBML and DDBJ databases. About 141 clones showed significant homology to genes in the databases. The majority of these ESTs (47) showed homology to human sequences and only a few (19) matched bovine sequence. 126 clones did not match database sequences and may represent novel transcripts.The EST sequencing approach has recently contributed tremendously to discover of new genes in several parasites. Thus, we performed the sequencing of 461 cDNA clones from the schizont stage of T.parva. The cDNA colnes were mapped to the 4-T.parva chromosomes and 20 of these clones were mapped to the 33 Sfil fragments of the T.parva genome. EST sequencing demonstrated its potential in the identification of new genes in this stage of the parasite and has contributed greatly to the addition of ESTs into the T.parva database. The sequence data reported is invaluable for T.parva genome mapping and in the comparative genome mapping of theileria parasites.

瑟氏泰勒虫是一种蜱传的牛原虫。良性牛泰勒虫寄生虫，据推测属于瑟氏泰勒虫/东方泰勒虫群，分布在亚洲国家。该寄生虫具有主要梨形体表面蛋白（MPSP）基因，MPSP基因是寄生虫逃避宿主免疫的手段之一。为了了解泰勒虫基因组图谱，利用表达序列标签（expressedsequencetag，EST）技术对牛泰勒虫转化T淋巴母细胞系后表达的基因进行了分析。对394个cDNA克隆进行单次测序，并在GeneBank、EBML和DDBJ数据库中搜索序列的显著同源性。约有141个克隆与数据库中的基因具有显著的同源性。这些EST中的大多数（47个）与人序列具有同源性，只有少数（19个）与牛序列相匹配。126个克隆与数据库序列不匹配，可能代表新的转录本。EST测序方法最近为发现几种寄生虫的新基因做出了巨大贡献。因此，我们进行了测序的461个cDNA克隆的小锥虫的鞭毛体阶段。将cDNA克隆定位到4-T.parva染色体上，其中20个克隆定位到T.parva基因组的33个Sfil片段上。EST测序证明了其在鉴定寄生虫这一阶段的新基因方面的潜力，并为将EST添加到小锥虫数据库中做出了巨大贡献。报道的序列数据对于小泰勒虫基因组作图和泰勒虫寄生虫的比较基因组作图是非常宝贵的。