Molecular evolution of Theileria parasites in Southern Asia

在日本，由梭形弧菌引起的牛螺旋体菌病是造成放牧牛经济损失的主要原因。sergentii / buffeli / orientalis群被称为牛的牛肠杆菌寄生虫，已知分布在世界各地。在此之前，我们开发了等位基因特异性聚合酶链反应（PCR），利用主要免疫显性引物的核苷酸序列设计的3组寡核苷酸引物，特异性扩增lach等位基因，这些引物来自两个t . sergenti stock, Ikeda(1)和Chitose c(1)和buffeli (B) stock, Warwick，的主要免疫显性piroplasm surface protein （p32）基因的核苷酸序列。并将该方法应用于南亚国家的野外调查。在两年中，我们从以下国家采集了血液样本：韩国（济州岛）、泰国、柬埔寨、印度、巴基斯坦、越南和中国。提取寄生虫DNA，采用等位基因特异性PCR对其进行分析。在韩国济州岛，所有分离株都含有I型寄生虫和C型寄生虫。而在泰国和柬埔寨，分布的主要寄生虫为泰国型寄生虫，与sergentii /buffeli / orientalis不同。在泰国和柬埔寨也分离到C型寄生虫和T、buffeli型寄生虫。在印度和巴基斯坦，主要的肠杆菌寄生虫被称为环状绦虫。我们期望能分离出良性的回肠菌，但是我们无法分离出良性的回肠菌。目前的资料表明，东亚和南亚国家的良性肠杆菌属寄生虫是混合寄生虫种群。

Bovine piropurasmosis due to Theileria sergenti is a major cause of economic loss in grazing cattle in Japan. T. sergentii / buffeli / orientalis grop is referred to as bengin theileria parasites of cattle and is known to be distributed all over the world. Previously, we developed the allele-specific polymerase chain reaction (PCR) which specifically amplifies lach allelic from using 3 sets of olgonucleotide primers designed from nucleotide sequences of major Immunodominant primers designed from nucleotide sequences of major Immunodominant piroplasm surface protein (p32) genes of two T. sergenti stocks, Ikeda (1) and Chitose c and one t, buffeli (B) stok, Warwick, and appled the method to the field survey of benign theileria parasites in southern asian countries.During 2 years, we have collected blood samples from the following countries : Korea (Cheju island), Thailand, Cambodia, India, Paklstan, Vietnam and China. Parasites DNA was extracted and the DNA was analyzed by using allele-specific PCR. In Korea (Cheju island), all the isolates contained I type parasites as well as C type parasites. However In Thailand and Cambodia, Major parasites distributed there countries were Thai type parasites, different from T. sergentii /buffeli / orientalis. C type parasites and T, buffeli type parasites were also isolated from Thailand and Cambodia. In India and Pakistan, major theileria parasites are known as T. annulata. We expected to isolate benign theileria parasites, however, we could not isolate benign theileria parasites. The present data showed that benign theileria parasites in east and Southern asian countries were mixed parasites populations.