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Analysis of tick-encoded immunoregulatory serine proteinase inhibitors (serpins)

蜱编码的免疫调节丝氨酸蛋白酶抑制剂（丝氨酸蛋白酶抑制剂）的分析

基本信息

批准号：
13460128
负责人：
ONUMA Misao
金额：
$ 9.92万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460128/
关键词：
serpin anti-tick vaccine immunoregulation recombinant antigen 吸血阻害

项目摘要

Ticks are obligate ectoparasites that infest mammals, birds, repltiles, and amphibians and are found in many region of the world where they are the major vectors transmitting a great number of pathogens of human and animals. In Japan and other East Asian countries, Haemaphisalis longicornis, a tick commonly infesting cattle and dogs is a major vector of Theileria sergenti/buffeli, which are economically important disease of cattle. In the livestock industry ticks, the importance of ticks is their role as vectors of disease pathogens of livestock. Suppression of the tick vector population is considered as the method of choice in the absence of vaccines to target specific diseases transmitted by ticks. Several disadvantages associated with the use of acaricide which is the current method of choice to control ticks have necessitated the need to develop alternative methods for the tick control. Host immunization against ticks is one of the most promising method whose success is dependent o … More n the identification and characterization of tick vaccine candidatesSerpins may represent one of the most interesting target antigens for tick vaccine development because of their role in regulation of several physiological functions such as the blood clotting cascade, clot resolution, the inflammatory response and complement activation. In humans, dysfunctions or deficiencies of serpins have resulted in diseases. Given the critical role that serpins play in physiology of several organisms, we proposed that ticks are likely to use serpins to disrupt defensive host processes and therefore could be considered as potential candidates far anti-tick vaccines. We have previously characterized two tick salivary gland proteins whose recombinant products were effective against tick. The focus of our research is to characterize additional protective antigens which can be used in cocktail with our previously characterized antigens. Recently, we succeed to the molecular cloning of H. longicomis serpin gene and the recombinant protein suggest the possible use of the tick vaccine. Less

蜱是专性体外寄生虫，寄生于哺乳动物、鸟类、复制动物和两栖动物，并且在世界许多地区发现，它们是传播人类和动物的大量病原体的主要媒介。在日本和其他东亚国家，长角血蜱（Haemaphisalis longicornis）是一种常见于牛和狗的蜱，是瑟氏泰勒虫（Theileria sergenti/buffeli）的主要媒介，瑟氏泰勒虫是牛的重要经济疾病。在畜牧业中，蜱的重要性在于它们作为牲畜疾病病原体的载体。在缺乏针对蜱传播的特定疾病的疫苗的情况下，抑制蜱媒种群被认为是首选方法。与作为当前控制蜱虫的选择方法的杀螨剂的使用相关的若干缺点使得需要开发用于蜱虫控制的替代方法。蜱的宿主免疫是最有前途的方法之一，其成功与否取决于宿主的免疫能力。 ...更多信息在蜱类疫苗候选物的鉴定和表征中，丝氨酸蛋白酶抑制剂可能是蜱类疫苗开发中最感兴趣的靶抗原之一，因为它们在调节几种生理功能如凝血级联反应、凝块消退、炎症反应和补体激活中起作用。在人类中，丝氨酸蛋白酶抑制剂的功能障碍或缺陷导致疾病。鉴于丝氨酸蛋白酶抑制剂在多种生物生理学中的重要作用，我们认为蜱很可能利用丝氨酸蛋白酶抑制剂破坏宿主的防御过程，因此可以被认为是抗蜱疫苗的潜在候选者。我们以前的特点是两个蜱唾液腺蛋白的重组产品是有效的抗蜱。我们研究的重点是表征额外的保护性抗原，其可以与我们先前表征的抗原混合使用。最近，我们成功地克隆了H.长角蜱丝氨酸蛋白酶抑制剂基因和重组蛋白的表达提示了长角蜱疫苗的可能性。少