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Biochemical and biological properties of tumor-associated antigen of bovine leukemia cells

牛白血病细胞肿瘤相关抗原的生化和生物学特性

基本信息

批准号：
61480085
负责人：
ONUMA Misao
金额：
$ 3.97万
依托单位：
Rakumo Gakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Enzootic bovine leukosis (EBL) is a lymphoproliferative disease caused by bovine leukemia virus (BLV). The neoplastic cells have tumor-associated antigens (TAA) on their cell surface. Thirteen monoclonal antibodies that identified TAA on EBL cells have been obtained. The properties of the TAA were analyzed by using these monoclonal antibodies. The results obtained in this studies are summerized as follows; 1. According to the reactivities of 13 monoclonal antibodies with 19 individual tumors, TAA were classified into 3 groups: common TAA, partially common TAA and individually distinct TAA. TAA bound to Lentil Lectin-Sepharose 4B column and were thus a glycoprotein. Common TAA with a molecular weight (M.w) of 74,000 (74K) had at least 2 independent antigenic regions coexisting on the molecule. The partially common TAA with a M.w of 64K had at least 4 independent antigenic regions. Individually distinct TAA with a M.w of 64K was located on molecule different from that of the partially common TAA. 2. The specificity of c143, recognized common TAA, binding in vivo for EBL tumor cells was demonstrated. The ^<125>I-labeled c143 F(ad')_2 fragments were injected into athymic mice bearing bovine lymphoid tumor cells. The fragments became preferentially localized in tumor tissues but not in normal tissues, as determined by radioimmunoscintigraphy. 3. An antitumor effect of c143 was tested. Nude mice injected with c143 (200<micrn>g) every other day until 37 days after the tumor transplantation showed significant inhibition of tumor growth when compared with control groups (p<0.05).

地方性牛白血病（EBL）是由牛白血病病毒（BLV）引起的一种淋巴组织增生性疾病。肿瘤细胞在其细胞表面上具有肿瘤相关抗原（TAA）。已获得13个鉴定EBL细胞上TAA的单克隆抗体。通过使用这些单克隆抗体分析TAA的性质。本研究的主要结果如下：1.根据13种单克隆抗体与19个肿瘤的反应性，将TAA分为3组：普通型TAA、部分普通型TAA和个体差异型TAA。TAA与小扁豆凝集素-琼脂糖4 B柱结合，因此是一种糖蛋白。分子量（M.w）为74，000（74 K）的常见TAA在分子上至少有两个独立的抗原区共存。部分共有的TAA分子量为64 K，至少有4个独立的抗原区。分子量为64 K的个体不同的TAA位于与部分共同的TAA不同的分子上。2.证实了c143（公认的常见TAA）在体内与EBL肿瘤细胞结合的特异性。将标记<125>的c143 F（ad '）_2片段注射到荷牛淋巴瘤细胞的裸鼠体内。碎片成为优先定位在肿瘤组织中，但不是在正常组织中，通过放射免疫闪烁法确定。3.测试了c143的抗肿瘤作用。与<micrn>对照组相比，每隔一天注射c143（200 μ g）直到肿瘤移植后37天的裸鼠显示出显著的肿瘤生长抑制（p<0.05）。