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Molecule gene analysis of bone absorption in bone reproduction medical care and development of bone absorption restraint medicine

骨再生医疗中骨吸收的分子基因分析及骨吸收抑制药物的开发

基本信息

批准号：
15390614
负责人：
SUGAHARA Toshio
金额：
$ 9.54万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390614/
关键词：
osteoprosis osteoclast Immunosuppresant agents 免疫柳制剤 T細胞 RANKL OPG リアルタイムPCR

项目摘要

Intramuscular injection (1 mg/kg) did immunity restraint agent FK506 to a nude mouse and a mouse of a wild type that a T cell suffered a loss day after day on 28th and made an osteoporosis model mouse to search a cell pro-immunity and relevance with the onset of osteoporosis by immunity restraint agent. As a result, as for the bone absorption of a group of experiments by FK506, decrease of trabecula bone was recognized by a wild type more underneath than enchondral ossification, and, as for positive osteoclast, numbers increased in enchondral ossification for TRAP dyeing more clearly than a group of control again. However, as for the bone absorption in the lower part, as for the osteoporosis by immunity restraint agent, what an immunity system cell such as a T cell was related to was suggested without most than enchondral ossification with a nude mouse by there not being control and a change.I discovered that osteoporosis recovered when we administered Propion acid bacteria fermentatio … More n product (PC) that was a starter of Swiss cheese to this osteoporosis model. We examined a bone absorption restraint effect from this fermentation product this time. I was able to prevent the bone absorption when I administered Propion acid bacteria fermentation product (PC) to an osteoporosis mouse by FK506.In late years a lot of existing DHNA has a naphthoquinone frame the same as vitamins K2 attracting attention in a quantity of bone salt thing having quantity of bone improvement action within a PC.I paid attention to this point, and we accepted bone absorption restraint same as a PC for an osteoporosis model when we administered DHNA alone. As for DHNA, the bone absorption restraint effect of a PC was able to identify a thing by DHNA from what was included in a PC abundantly.I compared osteoclast with control when I added the B cell which was a T cell and the B220 positive that were four twist CD positive in marrow in osteoclast of a mouse namely in vitro of bone bud cell-like strike Rome cell and marrow cell with magnetic beads and increased significantly. Less

28日肌肉注射免疫抑制剂FK506(1 mg/kg)对裸鼠和T细胞连续丢失的野生型小鼠进行免疫抑制，并用免疫抑制剂对骨质疏松模型小鼠进行细胞前免疫及与骨质疏松发病的相关性研究。结果表明，对于FK506实验的一组骨吸收，小梁骨的减少比软骨下骨化更能被野生型识别，而对于破骨细胞阳性，TRAP染色的软骨骨化组又比对照组更明显地增加了数量。然而，对于下部的骨吸收，对于免疫抑制剂引起的骨质疏松症，在没有对照和改变的情况下，建议与免疫系统的T细胞等免疫系统细胞有关，而不是通过裸鼠的软骨成骨。我发现当我们使用丙酸细菌发酵…时，骨质疏松症已经恢复更多的n产品(PC)是这个骨质疏松模型的瑞士奶酪的起始物。我们这次检测了这种发酵产品对骨吸收的抑制作用。我用FK506给骨质疏松小鼠注射丙酸菌发酵产物(PC)时，能够阻止骨吸收。近年来，许多现有的DHNA在PC内有大量具有骨改善作用的骨盐物质，具有与维生素K2相同的萘醌骨架，引起了人们的注意。我注意到了这一点，我们对骨质疏松模型采用了与PC相同的骨吸收抑制。对于DHNA，PC的骨吸收抑制作用能够充分从PC中包含的东西中识别出DHNA所包含的东西。我将破骨细胞与对照组进行比较，当我在小鼠破骨细胞中加入骨髓中四股CD阳性的B细胞(T细胞)和B220阳性细胞，即体外培养的骨芽细胞样撞击罗马细胞和带有磁珠的骨髓细胞时，破骨细胞明显增加。较少