The study of molecular and gene analysis and bone metabolism-improving effect of propiomibacterium freudenreichii milk whey culture against osteoporosis.

费氏丙酸杆菌乳清培养物抗骨质疏松的分子基因分析及骨代谢改善作用研究

• 批准号: 17390538
• 负责人: SUGAHARA Toshio
• 金额: $ 10.05万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390538/
• 关键词: Osteoporosis; Bifidobacteria; Propiomibacterium freudenreichii; DHNA; T-cell; B-cell; Naphthoquinone; Bone mass recovery; 免疫抑制剤; T, B細胞; FACS

To see the relationship between immune cell and osteoporosis occurrence by immunosuppresant, we have established the osteoporosis mouse model using immunosuppresant (FK506) injected nude mouse and wild type mouse. The results of wild type were amount of trabecula bone under enchondral ossification was decreased, and number of osteoclast, TRAP positive, was obviously increased in enchondral ossification. On the other hand, there was almost no bone resorption under enchondral ossification in the nude mouse' s result. Those results suggest that immune cell contributes to the osteoporosis occurrence by the immunosuppresant. We have found propiomibacterium freudenreichii milk whey culture (PC), starter of Swiss cheese, could rescue osteoporosis in our model. In further experiment, PC could inhibit the differentiation of osteoclast and prevent osteoporosis. But it is hard to simply say that PC rescued osteoporosis in our model by differential inhibition of osteoclast, because of the osteocla … More st differentiation and immune system participation. To see the details, we have gotten the following results, a) The number of osteoclast was significantly increased in the mouse osteoblast-like stroma cell and osteoblast mixed culture with supplementation of CD4 positive T-cell or B220 positive B-cell, extracted from bone marrow with magnet beads. b) FACS result was the immunosuppresant decreased the number of T-cell and increased the number of B-cell in spleen and mesentericlymph node of our model, c) DHNA, contained in PC, has naphthoquinone. The basic skeleton is the same as vitamin K2, which is a research attention of bone mass recovery in recent years. DHNA made the same results as PC's and was identified as a factor of inhibitory effect of bone resorption.We elucidated the bone mass recovery with DHNA, and the relationship immune system and osteoclast. Furthermore, to identify osteoporosis-related gene, we have seen the alteration of gene expression in'bone marrow with DNA microarray. Less

为了了解免疫抑制剂与骨质疏松发生的关系，我们采用免疫抑制剂FK506注射裸鼠和野生型小鼠建立骨质疏松小鼠模型。野生型软骨成骨时骨小梁数量减少，软骨成骨时TRAP阳性破骨细胞数量明显增加。另一方面，裸鼠内软骨成骨后几乎没有骨吸收。提示免疫细胞通过免疫抑制剂参与骨质疏松的发生。我们发现瑞士奶酪发酵剂丙杆菌乳清培养物（PC）可以挽救我们模型中的骨质疏松症。在进一步的实验中，PC可以抑制破骨细胞的分化，预防骨质疏松。但很难简单地说，在我们的模型中，PC通过对破骨细胞的差异抑制来拯救骨质疏松症，因为破骨细胞有更多的分化和免疫系统参与。具体来看，我们得到了以下结果：a)用磁珠提取骨髓，添加CD4阳性t细胞或B220阳性b细胞的小鼠成骨样基质细胞和成骨细胞混合培养中，破骨细胞的数量明显增加。b) FACS结果显示免疫抑制剂可使模型脾和肠系膜淋巴结t细胞数量减少，b细胞数量增加；c) PC中含有萘醌类dna。基本骨架与维生素K2相同，是近年来骨量恢复的研究热点。dna与PC的结果相同，被认为是抑制骨吸收的一个因素。我们阐明了dna对骨量恢复的影响，以及免疫系统与破骨细胞的关系。此外，为了鉴定骨质疏松相关基因，我们用DNA芯片观察了骨髓中基因表达的改变。少

项目成果

子どもは素晴らしい(お父さんとお母さんの子育てのために)

孩子们很棒（对于养育孩子的爸爸妈妈来说）

• 发表时间: 2006
• 作者: Shigeru;Maeda;Ichiro;Nakatsuka;Takuya;Miyawaki;Takuo;Kuboki;Masahiko;Shimada;Takashi Fujii;Tomohiro Yamada;Toshio Sugahara;Takaaki Ueno;Seiji Kondo;Seiji Kondo;Mishima K;Fujii T;Yamada T;Hitoshi Nagatsuka;Hitoshi Nagatsuka;菅原 利夫 共著
• 通讯作者: 菅原 利夫 共著

Various immunostaining patterns of CD31, CD34 and endoglin and their relationship with lymph node metastasis in oral squamous cell carcinomas

• DOI: 10.1111/j.1600-0714.2004.00227.x
• 发表时间: 2005-02-01
• 期刊: JOURNAL OF ORAL PATHOLOGY & MEDICINE
• 影响因子: 3.3
• 作者: Nagatsuka, H;Hibi, K;Nagai, N
• 通讯作者: Nagai, N

子どもは素晴らしい (お父さんとお母さんの子育てのために)

孩子们很精彩（因为爸爸妈妈的养育）

• 发表时间: 2006
• 作者: Shigeru;Maeda;Ichiro;Nakatsuka;Takuya;Miyawaki;Takuo;Kuboki;Masahiko;Shimada;Takashi Fujii;Tomohiro Yamada;Toshio Sugahara;Takaaki Ueno;Seiji Kondo;Seiji Kondo;Mishima K;Fujii T;Yamada T;Hitoshi Nagatsuka;Hitoshi Nagatsuka;菅原 利夫 共著;菅原 利夫 共著
• 通讯作者: 菅原 利夫 共著

Expression of transcription factor Sox9 in the cartilage formation from grafted periosteal cells.

转录因子 Sox9 在移植骨膜细胞软骨形成中的表达。

• 发表时间: 2006
• 期刊: Asian Journal Oral Maxillofac 18 (1)
• 作者: Satoru;Sakurai;Atsuo;F.;Fukunaga,Takuya;Miyawaki;Tatsuya;Ichinohe;Yuzuru;KanekoCalifornia;USA;Takaaki Ueno
• 通讯作者: Takaaki Ueno

Novel angiogenic inhibitor DN-9693 that inhibits Past-transcriptional induction of connective tissue growth factor (CTGF/CCN2) by vascular endothelial grow factor (VEGF) in human endothelial cells.

新型血管生成抑制剂 DN-9693 可抑制人内皮细胞中血管内皮生长因子 (VEGF) 对结缔组织生长因子 (CTGF/CCN2) 的转录诱导。

• 发表时间: 2006
• 期刊: Molecular Cancer Therapeutics 5
• 作者: Shigeru;Maeda;Ichiro;Nakatsuka;Takuya;Miyawaki;Takuo;Kuboki;Masahiko;Shimada;Takashi Fujii;Tomohiro Yamada;Toshio Sugahara;Takaaki Ueno;Seiji Kondo;Seiji Kondo
• 通讯作者: Seiji Kondo