A study to solve the mystery of AIDS pandemic by expeditions into the most remote areas in the Congo basin.

一项通过深入刚果盆地最偏远地区的探险来解开艾滋病大流行之谜的研究。

• 批准号: 22256001
• 负责人: IDO Eiji
• 金额: $ 29.2万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22256001/
• 关键词: ウイルス; HIV; 感染症; エイズ; 進化; アフリカ; コンゴ盆地; コンゴ民主共和国; 分子進化; コンゴ民主; コンゴ; 分子疫学; コンゴ共和国; 起源

The purpose of this study is to clarify the mystery of AIDS pandemic (when, where and how did it occur?) as well as the origin of HIV. To do so, we have expedited into far remote provinces in both Democratic Republic of Congo and Republic of Congo. In addition to the conventional molecular epidemiological techniquues, we also employed the so-called second generation high-throughput DNA sequencer. So far the areas showing the most diversified profiles of HIV genotypes are the central region of Congo basin and nearby areas the majority of which is covered with the giant Congo River and its numerous branch rivers. In those areas, not only a great variety of genotypes of HIV strains are co-circulating but also new types of viruses seem to be continuously generated with high probability by way of dual infection of two different genotypic HIV strains and recombination of their genomes as a natural consequent event.

这项研究的目的是澄清艾滋病大流行之谜（何时、何地和如何发生的？）以及艾滋病病毒的起源。为此，我们加快了对刚果民主共和国和刚果共和国偏远省份的调查。除了常规的分子流行病学技术外，我们还采用了所谓的第二代高通量DNA测序仪。到目前为止，艾滋病毒基因型分布最多样化的地区是刚果盆地的中部地区和附近地区，其中大部分被巨大的刚果河及其众多的分支河流所覆盖。在这些地区，不仅各种基因型的艾滋病毒株在共同传播，而且通过两种不同基因型的艾滋病毒株的双重感染及其基因组的重组作为自然结果，新型病毒似乎也在不断产生，而且这种可能性很高。

项目成果

A technique for capturing broad subtypes and circulating recombinant forms of HIV-1 based on anionic polymer-coated magnetic beads.

一种基于阴离子聚合物包被磁珠捕获广泛亚型和循环重组形式的 HIV-1 的技术。

• DOI: 10.3892/ijmm.2012.1009
• 发表时间: 2012
• 期刊: Int. J. Mol. Med.
• 作者: 新堀淳樹;辻 裕司;菊池 崇;荒木 徹;池田昭大;魚住禎司;R. E. S. Otadoy;歌田久司;B. M. Shevtsov;Baishev Dmitry;長妻 努;湯元清文;Sakudo A
• 通讯作者: Sakudo A

Establishment of in-house quantitative rea-time RT PCR assay for monitoring for HIV-1 viral loads and efficacy evaluation of ART in Ghanaian AIDS patients in an urban setting

建立内部定量实时 RT PCR 检测方法，用于监测城市环境中加纳艾滋病患者的 HIV-1 病毒载量和 ART 疗效评估

• 发表时间: 2011
• 作者: Barnor J;Brandful J;Ampofo W;Yamamoto N;Bonney K;Bonney M;Alale M;Ntim A;AmoahY;fori S;Addo A;Nyarko A;Ido E;Ishikawa K;Yamaoka S
• 通讯作者: Yamaoka S

Establishment of in-housequantative rea-time RT PCR assay for monitoring for HIV-1 viral loads and efficacyevaluation of ART in Ghanaian AIDS patients in an urban setting

建立内部定量实时 RT PCR 检测方法，用于监测城市环境中加纳艾滋病患者的 HIV-1 病毒载量和 ART 疗效评估

• 发表时间: 2011
• 作者: Barnor J;Brandful J;Ampofo W;Yamamoto N;Bonney K;Bonney M;Alale M;Ntim A;Amoah Y;fori S;Addo A;Nyarko A;Ido E;Ishikawa K;Yamaoka S
• 通讯作者: Yamaoka S

Molecular Evolution of HIV-1 CRF01_AE Env in Thai Patients

• DOI: 10.1371/journal.pone.0027098
• 发表时间: 2011-11-02
• 期刊: PLOS ONE
• 影响因子: 3.7
• 作者: Boonchawalit, Samatchaya;Jullaksorn, Duangrat;Kameoka, Masanori
• 通讯作者: Kameoka, Masanori

High-throughput DNA sequencing is a powerful tool to analyze dual and multiple HIV infections : its first application to the study in DRC

高通量 DNA 测序是分析双重和多重 HIV 感染的强大工具：首次应用于刚果民主共和国的研究

• 发表时间: 2013
• 作者: Ido E;Karhemere S;Ebengho M;Nakamura S;Nakaya T;Brandful J;Barnor J;Ampofo W;Yamaoka S;Muyembe JJ
• 通讯作者: Muyembe JJ