The production of a new selection system for probiotic lactic acid bacteria adhering to animal intestines and its application

首次采用BIACORE 1000 （Pharmacia）筛选动物肠道黏附性强的乳酸菌，建立了一种新的筛选方法。将鸡肠粘蛋白（CIM）包被在5型传感芯片上作为配体，对从鸡肠中分离的几种益生菌乳酸菌进行分析。用共振单位值（RU）评价粘附性。该方法成功地筛选出了几种细菌。对人肠的分析，采用两种商用生物素化聚合物探针（A型和B型）和人结肠粘蛋白（HCM）作为配体。还成功地分离出了几种益生菌。成功克隆了与HCM结合的干酪乳杆菌LA2表层蛋白中的一个凝集素，并明确了其核苷酸和氨基酸序列。我们展示了新选择的细菌在未来作为益生菌添加到食品中用于动物保健。

A new selection method by using BIACORE 1000 (Pharmacia) was first developed for the mass screening for lactic acid bacteria which strongly adhere to animal intestine.For the analysis of chicken intestine, chicken intestinal mucin (CIM) was coated to the sensor chip type 5 as ligand and investigated to several probiotic lactic acid bacteria which were isolated form chicken intestine as analyte. The adhesion was evaluated by resonance unit value (RU). Several bacteria were successfully selected by this method.For the analysis of human intestine, two types of commercial biotinilated polymer probe (blood type A and B) and human colon mucin (HCM) were used for ligand. Several probiotic bacteria were also successfully isolated.One lectin in the surface layer protein of Lactobacillus gasseri LA2 which was confirmed to combine to HCM was successfully cloned and clarified the nucleotide and amino acid sequence.We demonstrated the utilization of new selected bacteria for animal health by supplementing in food as probiotic bacteria in future.