Antihypertensive peptides without no side effect such as dry vomiting from milk proteins and their application

无奶蛋白干呕等副作用的降压肽及其应用

• 批准号: 14360158
• 负责人: SAITO Tadao
• 金额: $ 8万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14360158/
• 关键词: Milk protein; Epitope analysis; Angiotensin-II; AT1 receptor; Angiotensin-II receptor; Antihypertensive peptide; SHR; dry vomiting; 降圧ペプチド

The main purpose of this research is preparation of antihypertensive peptides without any side effects such as dry vomiting from milk proteins and their application for new functional foods and medicine.We constructed a new peptide library form commercial peptide products from DMV Company (Holland) which is very famous international company producing peptide of food additive grade. We isolated a tetra peptide : ALPM after hydrophobic-, ion-exchange chromatography and HPLC analysis. The peptide showed strong antihypertesivity by SHR (spontaneously hypertensive rats) and ACE inhibitory activities. The membrane expressing AT1 receptors were isolated from rat lungs. We tested the competitive inhibitory analysis with ALPM and I125labeled-angiotesin peptide II for the binding to AT1 receptor of the membrane. We first confirmed the inhibitory activity in ALPM and proposed the future application for the food or medicine.

本研究的主要目的是利用乳蛋白制备无干呕等副作用的降压肽，并将其应用于新型功能食品和药物。以荷兰DMV公司生产的食品添加剂级肽类产品为基础，构建了新的肽类库。经疏水、离子交换色谱和高效液相色谱分析，分离得到四肽ALPM。该肽对自发性高血压大鼠表现出较强的抗高血压作用和抑制ACE活性。从大鼠肺中分离出表达AT1受体的膜。我们测试了ALPM和i125标记的血管促素肽II对膜上AT1受体结合的竞争抑制分析。我们首先证实了其在ALPM中的抑制作用，并提出了未来在食品或药物中的应用。

项目成果

Tadao Saito: "Selection of useful probiotic lactic acid bacteria from the Lactobacillus acidophilus group and their applications to functional foods."Animal Science Journal. 75. 1-13 (2004)

Tadao Saito：“从嗜酸乳杆菌群中选择有用的益生菌乳酸菌及其在功能性食品中的应用。”《动物科学杂志》。

齋藤忠夫, 村上まり子, 殿内秀和: "ミルク・ヨーグルト・チーズで血圧を下げる!"畜産の研究. 57(5). 573-579 (2003)

Tadao Saito、Mariko Murakami、Hidekazu Tonouchi：“用牛奶、酸奶和奶酪降低血压！” 57(5) (2003)。

Tadao SAITO: "Selection of useful probiotic lactic acid bacteria from the L.acidophilus group and their applications to functional foods."Animal Science Journal. 75. 1-13 (2004)

Tadao SAITO：“从嗜酸乳杆菌群中选择有用的益生菌乳酸菌及其在功能性食品中的应用。”《动物科学杂志》。

齋藤忠夫: "一貫して「ミルク科学」の研究を中心に"牛乳生活情報. 31. 27-28 (2004)

Tadao Saito：“始终专注于‘牛奶科学’的研究”《牛奶生活方式信息》，31. 27-28 (2004)。

齋藤忠夫, 村上まり子, 殿内秀和: "ミルク・ヨーグルト・チーズで血圧を下げる!"畜産の研究. 57. 573-579 (2003)

Tadao Saito、Mariko Murakami、Hidekazu Tonouchi：“用牛奶、酸奶和奶酪降低血压！” 57. 573-579 (2003)。