Isolation of Immunostimulators from Bovine Colostrum and Its Chracteristic Analysis

牛初乳中免疫刺激剂的分离及其特性分析

• 批准号: 04660300
• 负责人: SAITO Tadao
• 金额: $ 1.34万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04660300/
• 关键词: Bovine colostrum; Immunostimulator; Affinity chromatography; Immunoglobulin G_1; Mitogenecity; B-Cell mitogen; Loctin-staining; Glucose-specific lectin; 免疫グロブリンG1

a. The specific binding proteinaceous components on Sephadex (D-gucose polymer) gels were isolated from bovine colostrum immediatedly after parturition. (Sephadex-affinity chromatography).b. The component was identified to immunoglobulin (Ig) G_1 by chemical analysis (SDS-polyacrylamide gel electrophoresis, high performance liquid chromatography and amino acid composition analysis) and immunological analysis (immunoelectrophoresis, Ouchterlony's gel diffusion analysis).c. Sephadex-binding (SB) IgG_1 showed strong mitogenic activity to mouse splenocytes. Especially, SB-IgG_1 only stimulated B-lymphocytes (B-cell mitogen) and the activity was lost after papain digestion.d. SB-IgG_1 strictly recognized alpha-D-Glucose (Glc) portion of oligosaccharides and the characteristic was originated from F_<ab> portion of its molecule.e. On lectin-like lstaining by biotinated SB-IgG_1, SB-IgG_1 strongly bound to hoth heavy chains from egg yolk antibody (IgY) and collagen which contained a glucosylated sugar chain.

一个。产后立即从牛初乳中分离出 Sephadex（D-葡萄糖聚合物）凝胶上的特异性结合蛋白质成分。 (Sephadex-亲和层析)。经化学分析（SDS-聚丙烯酰胺凝胶电泳、高效液相色谱、氨基酸组成分析）和免疫学分析（免疫电泳、Ouchterlony's凝胶扩散分析），鉴定该成分为免疫球蛋白（Ig）G_1。 Sephadex 结合 (SB) IgG_1 对小鼠脾细胞显示出强有丝分裂活性。特别是，SB-IgG_1仅刺激B淋巴细胞（B细胞有丝分裂原），木瓜蛋白酶消化后活性丧失。 SB-IgG_1严格识别寡糖的α-D-葡萄糖(Glc)部分，其特征源自其分子的F_<ab>部分。在通过生物素化 SB-IgG_1 进行凝集素样染色时，SB-IgG_1 与来自蛋黄抗体 (IgY) 和含有葡萄糖基化糖链的胶原蛋白的霍斯重链强烈结合。

项目成果

Makoto Miura: "Specific Absorption of Immunoglobulin G_1 in Bovine Colostrum on Sephadex and Its Mitogenic Activity on Mouse Splenocytes" Agric.Biol.Chem.55. 3103-3105 (1991)

Makoto Miura：“牛初乳中免疫球蛋白 G_1 在 Sephadex 上的特异性吸收及其对小鼠脾细胞的有丝分裂活性”Agric.Biol.Chem.55。

Tadao Saito et al: "Characateristics of Bovine Colostral Immunoglobulin G_1 with lectin-like and Mitogenic Activities" Anim. Sci. Technol.(Jpn.). 65. 105-111 (1993)

Tadao Saito 等人：“具有凝集素样和促有丝分裂活性的牛初乳免疫球蛋白 G_1 的特征”动画。

Makoto Miura et al: "Specific Adsorption of Immunoglobulin G_1 in Bovine Colostrum on Sephadex and Its Mitogenic Activity on Mouse Splenocytes" Agric. Biol. Chem.55. 3103-3105 (1991)

Makoto Miura 等人：“牛初乳中免疫球蛋白 G_1 在 Sephadex 上的特异性吸附及其对小鼠脾细胞的有丝分裂活性”Agric。

Tadao Saito: "Characteristics of Bovine Colostral Immunoglobulin G_1 with Lectin-like and Mitogenic Activities" Anim.Sci.Technol.(Jpn.). 65. 105-111 (1993)

Tadao Saito：“具有凝集素样和促有丝分裂活性的牛初乳免疫球蛋白 G_1 的特征”Anim.Sci.Technol.（日本）。

Makoto Miura: "Specific Adsorption of Immunoglobulin G_1 in Bovine Colostrum on Sephadex and Its Mitogenic Activity on Mouse Splenocytes" Agric.Biol.Chem.55. 3103-3105 (1991)

Makoto Miura：“牛初乳中免疫球蛋白 G_1 在 Sephadex 上的特异性吸附及其对小鼠脾细胞的有丝分裂活性”Agric.Biol.Chem.55。