Isolation of Immunostimulators from Bovine Colostrum and Its Chracteristic Analysis

a.从产后即刻的牛初乳中分离出葡聚糖凝胶上的特异性结合蛋白质组分。（Sephadex亲和层析）。经化学分析（SDS-聚丙烯酰胺凝胶电泳、高效液相色谱和氨基酸组成分析）和免疫学分析（免疫电泳、Ouchterlony凝胶扩散分析）鉴定为免疫球蛋白（IG）G_1。Sephadex结合（SB）IgG_1对小鼠脾细胞有较强的促有丝分裂活性。特别是SB-IgG_1只刺激B淋巴细胞（B细胞有丝分裂原），经木瓜蛋白酶消化后活性丧失。SB-IgG_1严格识别寡糖的α-D-葡萄糖（Glc）部分，其特征来源于<ab>其分子的F_1部分。生物素化SB-IgG_1凝集素样染色显示，SB-IgG_1与卵黄抗体（IgY）和含有糖基化糖链的胶原蛋白的重链有较强的结合。

a. The specific binding proteinaceous components on Sephadex (D-gucose polymer) gels were isolated from bovine colostrum immediatedly after parturition. (Sephadex-affinity chromatography).b. The component was identified to immunoglobulin (Ig) G_1 by chemical analysis (SDS-polyacrylamide gel electrophoresis, high performance liquid chromatography and amino acid composition analysis) and immunological analysis (immunoelectrophoresis, Ouchterlony's gel diffusion analysis).c. Sephadex-binding (SB) IgG_1 showed strong mitogenic activity to mouse splenocytes. Especially, SB-IgG_1 only stimulated B-lymphocytes (B-cell mitogen) and the activity was lost after papain digestion.d. SB-IgG_1 strictly recognized alpha-D-Glucose (Glc) portion of oligosaccharides and the characteristic was originated from F_<ab> portion of its molecule.e. On lectin-like lstaining by biotinated SB-IgG_1, SB-IgG_1 strongly bound to hoth heavy chains from egg yolk antibody (IgY) and collagen which contained a glucosylated sugar chain.