Integrated Analyses on adaptation and control mechanisms in remodeling of the extracellular matrix in the in situ frozen-thawed anterior cruciate ligament

原位冻融前交叉韧带细胞外基质重塑适应与控制机制综合分析

基本信息

  • 批准号:
    12470299
  • 负责人:
  • 金额:
    $ 9.15万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2002
  • 项目状态:
    已结题

项目摘要

1. This study biomechanically and molecular-biologically clarified the remodeling phenomenon of the patellar tendon (PT) and anterior cruciate ligament (ACL) tissues from the viewpoint of collagen synthesis and metabolism controlled by growth factors, using the in situ freeze-thaw treatment and several unique animal models which were originally developed by us. The results were shown as follows : (1) Intrinsic fibroblast necrosis did not significantly affect the mechanical properties of the PT, at least, for 6 weeks, but extrinsic fibroblast infiltration affected them. In addition, both stress deprivation and enhancement significantly deteriorated the mechanical properties of the in situ frozen ACL. (2) In the situ frozen PT, type-III procollagen mRNA was overexpressed in the extrinsic cells at 6 and 12 weeks, and type-III collagen was expressed around the extrinsic cells. (3) Application of IL-1beta and PDGF-BB enhanced expression of the collagenase mRNA in the extrinsic fibroblasts i … More n the frozen PT, while application of TGF-beta inhibited it. (4) Stress deprivation in the tendon tissue simultaneously induced not only overexpression of IL-1beta and TGF-beta-in fibroblasts but also reduction of the mechanical properties of the extracellular matrix. (5) A low-dose of TGF-β1 and EGF applied to the frozen-thawed ACL at 0 and 3 weeks inhibitd the material deterioration caused by freezing, but the same dose application at 6 weeks did not inhibit it. A high dose application did not inhibit it at each period. (6) Application of TGF-beta improves the material properties of healing tissue in the injured ACL, but application of the PDGF-BB does not have any effects.2. In addition, this study established a new integrated analysis system to clarify adaptation and control mechanisms in remodeling of the tendon and ligament tissue, using the biomechanical and molecular-biological methods. As to clinical relevance, this study demonstrated a basic principle of therapeutic trials to inhibit the deterioration of the material properties of the grafted tendon and ligament tissues, which is a significant clinical problem at the present time. We believe that this principle will contribute development of gene therapy and tissue engineering-based treatment for the injured tendon and ligament tissues in the field of orthopaedic surgery and sports medicine. Less
1.本研究从生长因子调控胶原合成和代谢的角度,采用原位冻融处理和我们自行开发的几种独特的动物模型,从生物力学和分子生物学角度阐明髌腱(PT)和前交叉韧带(ACL)组织的重塑现象。结果表明:(1)内源性成纤维细胞坏死对PT的力学性能无明显影响,至少在6周内,外源性成纤维细胞浸润对PT的力学性能有影响。此外,无论是应力剥夺和增强显着恶化的机械性能,在原位冷冻ACL。(2)在原位冷冻PT中,在6和12周时,III型前胶原mRNA在外源性细胞中过表达,并且III型胶原在外源性细胞周围表达。(3)应用IL-1 β和PDGF-BB促进体外培养的成纤维细胞胶原酶mRNA的表达 ...更多信息 (4)肌腱组织中的应力剥夺不仅诱导成纤维细胞中IL-1 β和TGF-β的过度表达,而且还导致细胞外基质的机械性能降低。(5)低剂量TGF-β1和EGF在冻融前交叉韧带0周和3周时均能抑制冻融后材料的降解,而6周时不能抑制冻融后材料的降解,高剂量时不能抑制冻融后材料的降解。(6)TGF-β的应用改善了损伤ACL中愈合组织的材料性质,但PDGF-BB的应用没有任何效果。此外,本研究还建立了一个新的综合分析体系,从生物力学和分子生物学的角度阐明肌腱和韧带组织改建的适应和控制机制。至于临床相关性,本研究证明了治疗试验的基本原则,以抑制移植肌腱和韧带组织的材料性能恶化,这是目前的一个重要临床问题。我们相信,这一原理将有助于发展基因治疗和组织工程为基础的治疗损伤的肌腱和韧带组织在骨科手术和运动医学领域。少

项目成果

期刊论文数量(288)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Fujiki H: "Change in contact conditions of knee joint caused by total knee and its effect"JSME. 43(4). 802-809 (2000)
藤木H:“全膝关节引起的膝关节接触条件的变化及其影响”JSME。
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  • 期刊:
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    0
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Tomita F: "Comparisons of intraosseous graft healing between the doubled flexor tendon graft and the bone-patellar tendon-bone graft in anterior cruciate ligament reconstruction"Arthroscopy. 17. 461-476 (2001)
Tomita F:“前交叉韧带重建中双屈肌腱移植物与骨-髌腱-骨移植物的骨内移植物愈合比较”关节镜。
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    0
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Tohyama H: "Extrinsic fibroblasts infiltrating the in situ frozen patellar tendon synthesize type-III collagen even when a physiological stress is applied to the tendon"Trans Orthop Res Soc. 26. 242-242 (2001)
Tohyama H:“即使对肌腱施加生理应力,浸润原位冷冻髌腱的外源成纤维细胞也会合成 III 型胶原”Trans Orthop Res Soc。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
Kudo FA: "Popliteal artery entrapment syndrome. Reconstruction of the transected medial head of the gastrocnemius muscle"J Cardiovasc Surg. 42. 817-819 (2001)
Kudo FA:“腘动脉卡压综合征。腓肠肌横切内侧头的重建”J Cardiovasc Surg。
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  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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冨田文久: "生体吸収性人工靭帯部を有する膝屈筋腱Hybrid代用材料の開発に関する基礎的検討"日本膝関節学会誌. 25. 174-178 (2001)
Fumihisa Tomita:“开发具有生物可吸收人工韧带的膝关节屈肌腱混合替代材料的基础研究”日本膝关节学会杂志 25. 174-178 (2001)。
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    0
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YASUDA Kazunori其他文献

YASUDA Kazunori的其他文献

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{{ truncateString('YASUDA Kazunori', 18)}}的其他基金

Flow analysis in a nanoscale space using scanning electron microscope and its development to soft matter flow
使用扫描电子显微镜进行纳米尺度空间流动分析及其对软物质流动的发展
  • 批准号:
    25630053
  • 财政年份:
    2013
  • 资助金额:
    $ 9.15万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Clarification of signal transmission mechanism in mesenchymal cells during spontaneous cartilage regeneration induced by multi-functional synthetic gel
多功能合成凝胶诱导自发软骨再生过程中间充质细胞信号传递机制的阐明
  • 批准号:
    23240070
  • 财政年份:
    2011
  • 资助金额:
    $ 9.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Visualization and analysis of soft materials in nanoscale flow for Development of nanocomposites
纳米级流动中软材料的可视化和分析,用于纳米复合材料的开发
  • 批准号:
    23656411
  • 财政年份:
    2011
  • 资助金额:
    $ 9.15万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Analyses on a molecular mechanism of spontaneous articular cartilage regeneration induced in vivo by double-network gel
双网络凝胶体内诱导关节软骨自发再生的分子机制分析
  • 批准号:
    20240045
  • 财政年份:
    2008
  • 资助金额:
    $ 9.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Visualization and analysis of flow-induced structure in micro flow for production of nanofiber composite
用于生产纳米纤维复合材料的微流中流致结构的可视化和分析
  • 批准号:
    20560641
  • 财政年份:
    2008
  • 资助金额:
    $ 9.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of flow-induced structure of polynieric fluid in a miatchannel for processing of nano composite materials
纳米复合材料加工微通道中多元流体的流动诱导结构分析
  • 批准号:
    18560667
  • 财政年份:
    2006
  • 资助金额:
    $ 9.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Clarification of Mechanism of the Tendon/Ligament Matrix Remodeling Induced by Various Mesenchymal Cells and Its Molecular Control
阐明多种间充质细胞诱导肌腱/韧带基质重塑的机制及其分子调控
  • 批准号:
    16390425
  • 财政年份:
    2004
  • 资助金额:
    $ 9.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Velocity measurement of velocity profile of complex fluids through a microchannel and the effect of the channel size on the flow pattern
复杂流体通过微通道的速度分布的速度测量以及通道尺寸对流型的影响
  • 批准号:
    15560141
  • 财政年份:
    2003
  • 资助金额:
    $ 9.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Visualization and numerical analysis of fiber orientation on weld line in processing for fiber-reinforced composite materials
纤维增强复合材料加工过程中焊缝纤维取向的可视化和数值分析
  • 批准号:
    13650747
  • 财政年份:
    2001
  • 资助金额:
    $ 9.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
DEVELOPMENT OF HYBRID-TYPE ARTIFICIAL LIGAMENT COMPOSED OF BIOABSORBABLE SYNTHETIC FIBERS AND HUMAN COLLAGEN BIBER BUNDLES
由生物可吸收合成纤维和人体胶原蛋白束组成的混合型人工韧带的开发
  • 批准号:
    10558124
  • 财政年份:
    1998
  • 资助金额:
    $ 9.15万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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细胞外基质对气道粘蛋白分泌的调节
  • 批准号:
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  • 财政年份:
    2023
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研究脱细胞组织细胞外基质凝胶的粘弹性特性,用于心脏和实质组织的治疗应用
  • 批准号:
    578501-2022
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    2022
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    RGPIN-2021-04259
  • 财政年份:
    2022
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    10895055
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An optical approach to 3-dimensional micro-mechanical imaging of the extra-cellular matrix (ECM)
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    DGECR-2021-00454
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    2021
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An optical approach to 3-dimensional micro-mechanical imaging of the extra-cellular matrix (ECM)
细胞外基质 (ECM) 3 维微机械成像的光学方法
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    10427422
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Elucidation of the Mechanism of Extra-Cellular-Matrix during Spontaneous Healing of Injured Anterior Cruciate Ligament and Development of the Accelerating Rehabilitation
前交叉韧带损伤自然愈合过程中细胞外基质机制的阐明及加速康复的发展
  • 批准号:
    21H03306
  • 财政年份:
    2021
  • 资助金额:
    $ 9.15万
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    Grant-in-Aid for Scientific Research (B)
An optical approach to 3-dimensional micro-mechanical imaging of the extra-cellular matrix (ECM)
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    10643828
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