Clarification of Mechanism of the Tendon/Ligament Matrix Remodeling Induced by Various Mesenchymal Cells and Its Molecular Control

1)应激剥夺显著增强了髌腱成纤维细胞中Mn-SOD、酪氨酸酶相关蛋白、KIAA1199相关蛋白、KIAA1199和pdgfr - α的基因表达。此外，Mn-SOD在m-RNA和蛋白水平上均在体内过表达。应激剥夺对髌腱il -1 β、tnf - α、tgf - β的表达也有显著增强作用，而对PDGF-B、bFGF的表达无显著影响。在移植肌腱的成纤维细胞中，VEGF在2周时过表达，在3周时达到峰值。2)来源于肌腱组织的外源性成纤维细胞（EFs）的增殖率显著低于肌腱内生性成纤维细胞（IFs）的增殖率。细胞培养后10 ~ 14天，上皮细胞的浸润率明显高于上皮细胞。两种成纤维细胞体外浸润到髌骨肌腱显著降低了筋膜的力学性能。3)应用tgf - β可显著减轻应力剥夺引起的髌腱力学性能恶化，而应用抗tgf - β抗体可显著增强应力剥夺引起的髌腱力学性能恶化。对于原位冻融前交叉韧带和移植物髌骨肌腱，tgf - β 1应用无明显影响。对于髌腱缺损再生的纤维组织，tgf - β 1的应用显著提高了其力学性能。VEGF应用于原位冻融前交叉韧带可显著促进血管再生，但对力学性能无明显影响。4)在补充tgf - β 1的培养基中培养的滑膜组织源性细胞显著提高了ACL重建中移植肌腱的力学性能和髌腱缺损中再生的纤维组织。少

1)Stress deprivation significantly enhanced gene expression of Mn-SOD, Tyrosinase-related protein, KIAA1199-related protein, KIAA1199, and PDGFR-alpha in fibroblasts in the patellar tendon. In addition, Mn-SOD was over-expressed in vivo at both the m-RNA and protein levels. Stress deprivation also significantly enhanced expression of Expression of IL-1beta, TNF-alpha, and TGF-beta in the patellar tendon, while it provided no significant effects to expression of PDGF-B and bFGF. In fibroblasts of the grafted tendon, VEGF was over-expressed at 2 weeks and showed a peak at 3 weeks.2)The proliferation rate of extrinsic fibroblasts (EFs) derived from the tendon tissue was significantly lower was significantly lower than that of intrinsic fibroblasts (IFs) in the tendon. The infiltrative rate was significantly higher in EFs than in IFs on 10 to 14 days after cell culture. Ex vivo infiltration of both fibroblasts into the patellar tendon significantly reduced the mechanical properties of fasc … More icles from the tendon.3)An application of TGF-beta1 significantly reduced the deterioration of mechanical properties of the patellar tendon caused by stress deprivation, while an application of anti-TGF-beta antibody significantly enhanced the degradation. Concerning the in situ frozen-thawed anterior cruciate ligament and the grafted patellar tendon, the TGF-beta1 application did not show' any significant effects. Regarding a fibrous tissue regenerated in a defect created in the patellar tendon, the TGF-beta1 application significantly increased the mechanical properties. An application of VEGF to the in situ frozen-thawed ACL significantly enhanced vascular regeneration, while it did not significantly affect the mechanical properties.4)An application of synovial tissue-derived cells cultured in TGF-beta1-suplimented medium significantly increased the mechanical properties of the grafted tendon in ACL reconstruction and the fibrous tissue regenerated in a defect created in the patellar tendon. Less