Clarification of Mechanism of the Tendon/Ligament Matrix Remodeling Induced by Various Mesenchymal Cells and Its Molecular Control

1)应激剥夺显著增强了成纤维细胞中Mn-SOD、酪氨酸酶相关蛋白、KIAA1199相关蛋白、KIAA1199和PDGFR-α的基因表达。此外，在体内的m-RNA和蛋白质水平上，Mn-SOD均有过表达。应激剥夺还显著增加了IL-1β、TNF-α和TGF-β在髌腱中的表达，而对PDGF-B和bFGF的表达无明显影响。2)肌腱组织来源的外源性成纤维细胞(EFS)的增殖率明显低于肌腱内成纤维细胞(IF)。在细胞培养后10~14d，EFS组的浸润率明显高于IF组。两种成纤维细胞在体外对髌腱的渗透显著降低了Fasc…的力学性能3)应用转化生长因子-β1显著减轻了应力剥夺引起的髌腱力学性能的恶化，而应用抗转化生长因子-β抗体则显著促进了该降解。对于原位冻融的前交叉韧带和移植的髌腱，应用转化生长因子-β1没有显示出任何明显的效果。对于在髌腱缺损处再生的纤维组织，应用转化生长因子-β1显著提高了力学性能。在原位冻融前交叉韧带中应用血管内皮生长因子可显著促进血管再生，但对力学性能无明显影响。4)在含有转化生长因子-β1的培养液中培养的滑膜组织衍生细胞显著提高了移植肌腱在重建前交叉韧带中的力学性能，并在髌腱形成的缺损处再生纤维组织。较少

1)Stress deprivation significantly enhanced gene expression of Mn-SOD, Tyrosinase-related protein, KIAA1199-related protein, KIAA1199, and PDGFR-alpha in fibroblasts in the patellar tendon. In addition, Mn-SOD was over-expressed in vivo at both the m-RNA and protein levels. Stress deprivation also significantly enhanced expression of Expression of IL-1beta, TNF-alpha, and TGF-beta in the patellar tendon, while it provided no significant effects to expression of PDGF-B and bFGF. In fibroblasts of the grafted tendon, VEGF was over-expressed at 2 weeks and showed a peak at 3 weeks.2)The proliferation rate of extrinsic fibroblasts (EFs) derived from the tendon tissue was significantly lower was significantly lower than that of intrinsic fibroblasts (IFs) in the tendon. The infiltrative rate was significantly higher in EFs than in IFs on 10 to 14 days after cell culture. Ex vivo infiltration of both fibroblasts into the patellar tendon significantly reduced the mechanical properties of fasc … More icles from the tendon.3)An application of TGF-beta1 significantly reduced the deterioration of mechanical properties of the patellar tendon caused by stress deprivation, while an application of anti-TGF-beta antibody significantly enhanced the degradation. Concerning the in situ frozen-thawed anterior cruciate ligament and the grafted patellar tendon, the TGF-beta1 application did not show' any significant effects. Regarding a fibrous tissue regenerated in a defect created in the patellar tendon, the TGF-beta1 application significantly increased the mechanical properties. An application of VEGF to the in situ frozen-thawed ACL significantly enhanced vascular regeneration, while it did not significantly affect the mechanical properties.4)An application of synovial tissue-derived cells cultured in TGF-beta1-suplimented medium significantly increased the mechanical properties of the grafted tendon in ACL reconstruction and the fibrous tissue regenerated in a defect created in the patellar tendon. Less