Clarification of Mechanism of the Tendon/Ligament Matrix Remodeling Induced by Various Mesenchymal Cells and Its Molecular Control

阐明多种间充质细胞诱导肌腱/韧带基质重塑的机制及其分子调控

• 批准号: 16390425
• 负责人: YASUDA Kazunori
• 金额: $ 8.83万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390425/
• 关键词: Soft tissues; Tendon; Ligament; Matrix; Remodeling; Fibroblast; Stress deprivation; Molecular control; 徐負荷; 靱帯

1)Stress deprivation significantly enhanced gene expression of Mn-SOD, Tyrosinase-related protein, KIAA1199-related protein, KIAA1199, and PDGFR-alpha in fibroblasts in the patellar tendon. In addition, Mn-SOD was over-expressed in vivo at both the m-RNA and protein levels. Stress deprivation also significantly enhanced expression of Expression of IL-1beta, TNF-alpha, and TGF-beta in the patellar tendon, while it provided no significant effects to expression of PDGF-B and bFGF. In fibroblasts of the grafted tendon, VEGF was over-expressed at 2 weeks and showed a peak at 3 weeks.2)The proliferation rate of extrinsic fibroblasts (EFs) derived from the tendon tissue was significantly lower was significantly lower than that of intrinsic fibroblasts (IFs) in the tendon. The infiltrative rate was significantly higher in EFs than in IFs on 10 to 14 days after cell culture. Ex vivo infiltration of both fibroblasts into the patellar tendon significantly reduced the mechanical properties of fasc … More icles from the tendon.3)An application of TGF-beta1 significantly reduced the deterioration of mechanical properties of the patellar tendon caused by stress deprivation, while an application of anti-TGF-beta antibody significantly enhanced the degradation. Concerning the in situ frozen-thawed anterior cruciate ligament and the grafted patellar tendon, the TGF-beta1 application did not show' any significant effects. Regarding a fibrous tissue regenerated in a defect created in the patellar tendon, the TGF-beta1 application significantly increased the mechanical properties. An application of VEGF to the in situ frozen-thawed ACL significantly enhanced vascular regeneration, while it did not significantly affect the mechanical properties.4)An application of synovial tissue-derived cells cultured in TGF-beta1-suplimented medium significantly increased the mechanical properties of the grafted tendon in ACL reconstruction and the fibrous tissue regenerated in a defect created in the patellar tendon. Less

1）应激剥夺显着增强了Mn-SOD，酪氨酸酶相关蛋白，KIAA1199相关蛋白，KIAA1199和PDGFR-Alpha的基因表达，在pat子腱中成纤维细胞中。此外，在M-RNA和蛋白质水平上，Mn-SOD在体内过表达。压力剥夺还显着增强了pat肌肌腱中IL-1BETA，TNF-ALPHA和TGF-β表达的表达，而对PDGF-B和BFGF的表达没有显着影响。在移植肌腱的成纤维细胞中，VEGF在2周时过度表达，并在3周时显示出峰值。2）肌腱中内胶质成纤维细胞（IFS）的外在成纤维细胞（EFS）的增殖速率显着降低了。在细胞培养后10至14天，EFS中EFS的浸润率显着高于IFS。两种成纤维细胞在put骨肌腱中的体内浸润显着降低了肌腱的机械性能……3）TGF-BETA1的应用显着降低了patellar肌腱机械性能的降低，而剥夺了patellar肌腱的机械性能，而由抗应力造成的抗tgf-bet-beta antiby a antbody a antbody则增强了DERGRADY的应用。关于原位冷冻透射的前交叉韧带和移植的pat肌肌腱，TGF-BETA1应用没有显示出“任何显着影响”。关于在pat肌肌腱中产生的缺陷中再生的纤维组织，TGF-BETA1的应用显着提高了机械性能。 VEGF在原位冻结的ACL中的应用显着增强了血管再生，而它并未显着影响机械性能。4）在TGF-BetA1化合物中培养的滑膜组织衍生细胞的应用，在ACL重新构造中的机械性能中，在TGF-BETA1进行的培养基中均具有显着提高的机械性能，并增加肌腱。较少的

项目成果

Local administration of synovium-derived fibroblasts cultured in TGF-betal-supplemented medium inhibits reduction of structural properties of the tendon autograft after anterior cruciate ligament reconstruction : A sheep model study.

局部施用在添加 TGF-β 的培养基中培养的滑膜来源的成纤维细胞可抑制前十字韧带重建后自体肌腱结构特性的降低：一项绵羊模型研究。

• 发表时间: 2007
• 期刊: Trans Orthop Res Soc 32
• 作者: Tsuda;M.;et al.;Ikema Y;Kondo E;Hayashi R;Miyatake S;Okamoto S;Ikema Y.;Kondo E.
• 通讯作者: Kondo E.

Temporal changes in relationship between fibroblast repopulation, VEGF expression and angiogenesis in the patellar tendon graft after anterior cruciate ligament reconstruction

前交叉韧带重建后髌腱移植物中成纤维细胞再生、VEGF表达和血管生成之间关系的时间变化

• 发表时间: 2004
• 期刊: Trans Orthop Res Soc 29
• 作者: Ikeda;T;Ikeda T;Kondo E;Tohyama H;Ikema Y;Kondo E;Katsura T;Uchida H;Tohyama H.;Kondo E.;Yoshikawa T.;Katsura T.;Uchida H;Anaguchi Y;Yamazaki S;Ikema Y;Tohyama H;Kondo E;Kudo T;Yasuda K;Ikema Y;Yoshikawa T
• 通讯作者: Yoshikawa T

Reduction of initial tension in the in situ frozen anterior cruciate ligament.

减少原位冷冻前十字韧带的初始张力。

• 发表时间: 2004
• 期刊: Clin Orthop Relat R 419
• 作者: Ikeda;T;Ikeda T;Kondo E;Tohyama H;Ikema Y;Kondo E;Katsura T;Uchida H;Tohyama H.;Kondo E.;Yoshikawa T.;Katsura T.;Uchida H;Anaguchi Y;Yamazaki S;Ikema Y;Tohyama H;Kondo E;Kudo T;Yasuda K;Ikema Y;Yoshikawa T;Katsura T;Kondo E;桂 太郎;Mlkami S.
• 通讯作者: Mlkami S.

mechanical properties of the anterior cruciate ligament treated with radiofrequency shrinkage are deteriorated in vivo with time, even when sufficient volume of ligament tissue remains intact around the treated portion

即使治疗部分周围有足够数量的韧带组织保持完整，经射频收缩治疗的前交叉韧带的机械性能也会随着时间的推移而在体内恶化

• 发表时间: 2004
• 期刊: Trans Orthop Res Soc 29
• 作者: Ikeda;T;Ikeda T;Kondo E;Tohyama H;Ikema Y;Kondo E;Katsura T;Uchida H;Tohyama H.;Kondo E.;Yoshikawa T.;Katsura T.;Uchida H;Anaguchi Y;Yamazaki S;Ikema Y;Tohyama H;Kondo E;Kudo T;Yasuda K;Ikema Y;Yoshikawa T;Katsura T;Kondo E
• 通讯作者: Kondo E

Local administration of synovium-derived fibroblasts cultured in TGF-beta1- supplemented medium inhibits reduction of structural properties of the tendon autograft after anterior cruciate ligament reconstruction : A sheep model study

局部施用在补充 TGF-β1 的培养基中培养的滑膜来源的成纤维细胞可抑制前十字韧带重建后自体肌腱结构特性的降低：一项绵羊模型研究

• 发表时间: 2007
• 期刊: Trans Orthop Res Soc 32
• 作者: Tsuda;M.;et al.;Ikema Y;Kondo E
• 通讯作者: Kondo E